注射用丹参多酚酸对周细胞增殖与迁移和凋亡的影响

目的 探讨注射用丹参多酚酸对周细胞(C3H/10T1/2)增殖、迁移和凋亡的影响.方法 C3H/10T1/2细胞采用氧糖剥夺/复氧(OGD/R)模型模拟缺血缺氧损伤.将细胞分为5组:正常组、模型组和低、中、高3个剂量的实验组(注射用丹参多酚酸的6.25,12.50,25.00μg·mL-1),正常组将培养液置换为新鲜的...

Effect of Salvianolic acid for injection on the proliferation,migration and apoptosis of pericytes in vitro

Objective To investigate the effects of Salvianolic acid for injection on the proliferation,migration and apoptosis of pericytes(C3 H/10 T1/2).Methods Oxygen-glucose deprivation/reoxygenation(OGD/R)model was used to simulate ischemic and anoxic injury in cells.The cells were divided into 5 groups:normal group,model group and experimental-L,-M,-H groups(Salvianolic acid for injection:6.25,12.50,25.00μg·mL^-1).The normal group replaced the culture medium with fresh high Sugar DMEM,6 h later,cultured in the box for 18 h;the model group replaced the culture medium with sugar-free DMEM,after 6 h,changed to high-sugar medium,cultured in the box for 18 h;the experimental group was replaced with medicine-containing after 6 h on the basis of the model group incubate for 18 h in a high-sugar medium incubator.Cell counting kit-8 method was used to detect the proliferation level.The pericyte migration was detected by the cell scratch test.The expression levels of apoptosis-related proteins B cell lymphoma/lewkmia-2(Bcl-2)and Bcl-2-associated X protein(Bax)were detected by Western blot.Results The cell viability in normal group,model group,and low,medium,high dose experimental groups were(100.00±8.18)%,(80.49±6.79)%,(98.36±9.65)%,(98.92±9.05)%and(103.10±8.73)%,respectively;the migration rates in the 5 groups were(44.32±8.78)%,(26.96±2.78)%,(38.24±1.74)%,(41.13±1.88)%and(49.92±2.18)%,respectively;the expression level of Bcl-2 protein in the 5 groups were 100%,(41.95±9.86)%,(109.73±9.00)%,(91.14±9.49)%and(82.14±10.36)%;and the expression level of Bax protein in the 5 groups were 100%,(131.61±4.60)%,(67.26±0.99)%,(85.17±14.35)%and(93.39±14.00)%.Comparison between model group and normal group,or comparison between experimental-L,-M,-H groups and model group,the difference of the factors were significant(P<0.05,P<0.01).Conclusion Salvianolic acid for injection can promote the cell proliferation and cell migration ability of OGD/R injured pericytes and inhibit cell apoptosis.This study provides a basis for the clinical application of the protective effect of Salvianolic acid for injection on cerebral ischemia.