CXCR4/SDF-1反应轴与VEGF在强直性脊柱炎患者外周血单个核细胞中的表达及其相关性分析

［摘要］ 目的：探讨CXC趋化因子受体4/基质细胞衍生因子-1(CXCR4/SDF-1)反应轴与血管内皮细胞生长因子（VEGF）在强直性脊柱炎（AS）发病中的作用及其作用机制，阐明VEGF作为AS活动期和监测预后指标的可行性。方法：采用流式细胞术（FCM）检测30例AS患者及20例健康对照者外周血单个核细胞（PBMCs）CXCR4的表达情况；酶联免疫吸附法（ELISA）检测2组研究对象血清中的VEGF及SDF-1的表达水平；对CXCR4、SDF-1和VEGF三者间及其与疾病活动性指标血沉（ESR）和C反应蛋白（CRP）相关性进行分析。结果：AS组患者CXCR4表达阳性率显著高于健康对照组（P<0.05）；AS组患者VEGF及SDF-1的表达水平较健康对照组均明显增高（P<0.05）；CXCR4、SDF-1和VEGF三者间呈正相关关系，其相关系数(r)分别为0.828、0.638和0.723（P<0.05）；VEGF与ESR和CRP呈明显正相关关系，r分别为0.584和0.772（P<0.05）；CXCR4及SDF-1与ESR和CRP未见明显相关关系（P> 0.05）。结论：CXCR4/SDF-1反应轴与VEGF对AS发病、病情进展、严重程度及预后判断具有重要的作用；VEGF可作为活动期和监测预后的可靠指标，三者可能成为AS靶向治疗的主要靶点。

Expressions of CXCR4/SDF-1 reaction axis and VEGF in peripheral blood mononuclear cells in patients with ankylosing spondylitis and their correlation analysis

Objective To explore the effects of CXCR4 / SDF-1 axis and the VEGF on pathogenesis of ankylosing spondylitis(AS) and their related mechanisms,and to clarify the reliability of using VEGF as the index of active stage and monitoring prognosis of AS.Methods Flow cytometry(FCM) was used to detect the expression of CXCR4 on peripheral blood mononuclear cells(PBMCs) of 30 AS patients and 20 healthy persons.Enzyme linked immunosorbent assay(ELISA) was used to determine the serum VEGF and the SDF-1 levels in two groups.Relationship among CXCR4,SDF-1 and VEGF,and the relationship between above three indexes and disease activity indexes such as erythrocyte sedimentation rate(ESR) and C reactive protein(CRP) were analyzed.Results The positive expression rate of CXCR4 of the patients in AS group was significantly higher than that in control group(P<0.05).The serum VEGF and SDF-1 levels of the patients in AS group were significantly higher than those in control group(all P<0.05).There were positive correlations among the CXCR4,SDF-1 and VEGF(r=0.828,r=0.638,r=0.723,all P<0.05).There were positive correlations between the serum VEGF level and the levels of ESR and CRP(r= 0.584,r=0.723,all P<0.05).But there was no obvious correlation between the CXCR4,SDF-1 serum level and the levels of ESR and CRP(all P>0.05).Conclusion CXCR4/SDF-1 response axis and VEGF may have decisive roles in estimating the occurrence,development,severity and prognosis of AS;VEGF can be as reliable index of active stage and monitoring prognosis of AS,while those three may be the principal targets of targeting therapy.