西兰花多肽对C6胶质瘤细胞的诱导凋亡作用

目的：研究西兰花多肽对大鼠C6胶质瘤细胞生长的影响，初步探讨西兰花多肽对肿瘤细胞的诱导凋亡作用。方法：提取、分离及纯化西兰花多肽；培养大鼠C6胶质瘤细胞，用不同剂量（0、0.01、0.10、1.00和10.00 mg?L^-1）西兰花多肽作用24 、48和72 h后，应用倒置显微镜观察细胞凋亡情况，MTT方法检测药物作用后细胞的生长情况。结果：西兰花粗多肽有4个主要洗脱峰，其中第2峰收集液多肽（组分Ⅱ）含量最高，用于后续活性研究。西兰花多肽作用大鼠C6胶质瘤细胞后，倒置显微镜下观察到明显的凋亡小体，尤其以西兰花多肽10.00 mg?L^-1组最为明显。MTT结果，药物作用48 h时，西兰花多肽10 mg?L^-1组细胞增殖活性（0.127±0.011）明显降低，与对照组（0.501±0.035）比较差异有统计学意义（P<0.01）。药物作用72 h时，西兰花多肽各剂量组均表现为对细胞的抑制作用，与对照组（0.753±0.022）比较，细胞增殖活性西兰花多肽1.00 mg?L-1组（0.583±0.082）和10.00 mg?L^-1组（0.073±0.001）明显降低（P<0.01）。结论：西兰花多肽可诱导C6胶质瘤细胞发生凋亡反应，并且随着药物剂量升高和作用时间延长，凋亡效应增强，提示西兰花多肽具有良好的诱导肿瘤细胞凋亡的作用。

Induction of broccoli polypeptide on apoptosis of C6 glioma cells

Objective To study the effects of broccoli polypeptide on the proliferation of C6 glioma cells of rats,and to discuss the induction of broccoli polypeptide on apoptosis of tumor cells.Methods The crude broccoli peptide was ectracted,separated and purificated.The rat C6 glioma cells were cultivated and treated with different doses of broccoli polypeptide(0,0.01,0.10,1.00,10.00 mg·L-1) for 24,48 and 72 h,and the apoptosis of cells was observed under inverted microscope,then the growth of C6 glioma cells after treated with broccoli polypeptide was detected by MTT assay.Results Broccoli polypeptide had four main eluting peaks,and the second one(partⅡ) was rich in polypeptide which was used for continued study.The apoptotic bodies were found under inverted microscope after the C6 glioma cells were treated with broccoli polypeptide,especially in 10.00 mg·L-1 group.The MTT results showed that after treated for 48 h,the proliferation ability of cells in 10 mg·L-1 group(0.127±0.011) was significantly decreased compared with control group(0.501±0.035,P<0.01).After treated for 72 h,all doses of polypeptide inhibited the growth of cells;compared with control group(0.753±0.022),the proliferation abilities of the cells in 1.00 mg·L-1 group(0.583±0.082) and 10.00 mg·L-1 group(0.073±0.001) were significantly decreased(P<0.01).Conclusion Broccoli polypeptide may induce the apoptosis of C6 glioma cells,and the effect is increased with the prolengation of the treatment time and the increasing of drug dose,which suggesting that broccoli polypeptide can induce the apoptosis of tumor cells.