流式细胞术检测斑蝥酊剂发泡液中的成分研究

目的:斑蝥酊剂可以引起皮肤产生水泡,通过检测发泡液中的成分从而研究斑蝥的发泡作用对机体免疫调节的影响。方法:斑蝥酊剂作用于皮肤后8-12 h产生水泡,48 h后抽取发泡液,并用流工细胞术分析发泡液中的细胞组分。结果:发泡液大约有1×106-1×107/ml的细胞。其中大部分是中性粒细胞,巨噬细胞,还有树突状细胞(DC),而且发泡液的DC是外周血DC的3-10倍,并且还有Th1细胞因子IL-12的分泌。结论:通过斑蝥的发泡作用可以局部富集DC并促进DC的成熟和分化,诱导Th1细胞因子的分泌,活化T细胞,并从局部到全身,激发人体机能的自身调节,提高机体的免疫功能,并为通过发泡疗法治疗传染性疾病提供新的实验依据。

A Technique for Investigating the Component of Cantharidin Blisters by Flow Cytometry

OBJECTIVE: Cantharidimide cause blister. The effect of blister on immunoregulation was investigated. METHODS: Cantharidimide was placed on the skin, 48h later, the blister was analyzed by flow cytometry. RESULTS: The blister contained 1 x 10(6) - 1 x 10(7) cells per ml, most of which were neutrophils, macrophages, dendritic cells (DC), and IL-12 secreted by Thl cells. CONCLUSION: There are high concent of DC in the blister, which is differential and induce the secretion of Th1, the activation of T cell. The blister modulate the biological response of patients and is helpful for treatment with infective disease.