亚甲兰对人肺癌细胞(LTEP-a_3)的体外杀伤作用的研究

用直接杀伤,细胞生长的抑制及克隆形成能力的抑制等方法,试验了亚甲兰体外的抗肿瘤效应,发现其抗瘤作用有剂量及时间依赖性两种特征1μg/ml亚甲兰处理4小时,从肺腺癌细胞LTEP—a_3克隆存活为对照的10^(-1),48小时为10^(-2)72小时为10^(-3),亚甲兰浓度在1μg/ml以以上,延长作用时间显著增强了其对癌细胞的杀伤力,当处理时间在24小时或为上时,杀伤效应与浓度相关,亚甲兰对a_3癌细胞主要损伤部位为线粒体。

ANTITUMOR EFFECT OF METHYLENE BLUE!N VITRO

Antitumor effect of methylene blue in vitro were tested by methods of directkilling and inhibition of cell growth and clonogenic survival, Both dose and timedependent were the main character of It's antitumor efficiency, The surviving frac-tion of human lung adenocarcinoma cell line LTEP-a3 at concentration of 1 ug/ml of this dye treating for 4 hours was 10^-1,48 hours 10^-2,72 hours 10^-3,prolong-ing treating time at concentration of 1 ug/ml or more of methylene blue could inc-rease it's killing efficiency for cancer cells obviously, When the btreating timewere prolonged to 24 hours or more, the killing efficiency were related closelyto Its concentration, The damage location of cancer cells by meihylene blue wereobserved mainly in- mitochondria.