| 牛磺酸对锰致海马神经元损伤影响 |
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| 引用本文： | 姚雪婷,郭松超.牛磺酸对锰致海马神经元损伤影响[J].中国公共卫生,2011,27(12). |
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| 作者姓名： | 姚雪婷 郭松超 |
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| 作者单位： | 1. 广西壮族自治区疾病预防控制中心食品营养安全与学校卫生所,南宁,530028
2. 广西医科大学实验动物中心 |
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| 基金项目： | 国家自然科学基金(30660156) |
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| 摘 要： | 目的 探讨牛磺酸对锰致海马神经元损伤的影响.方法 新生Wistar大鼠海马神经元细胞原代培养后随机分为空白组、牛磺酸组、低、中、高剂量锰组、牛磺酸+低、中、高剂量锰组,检测乳酸脱氢酶(LDH)活力、流式细胞仪定量凋亡率和观察神经元超微结构变化.结果 LDH活力低、中、高剂量锰组分别为(1 048.063 0±55.459 8)、(1 004.1233±65.339 4)、(1 405.303 3±163.311 5)U/L,明显高于空白组(895.203 3±53.228 6) U/L(F=31.802,P＜0.001),牛磺酸+低剂量锰组(834.595 0±173.445 5)U/L明显低于低剂量锰组(1 048.063±55.459 8) U/L(t=2.871,P=0.017)；神经元凋亡率中、高剂量锰组分别为(44.6±4.0)％和(57.7±6.2)％,均明显高于空白组(17.5±2.5)％ (F=159.05,P＜0.001),牛磺酸+中剂量锰组(34.6±6.4)％明显低于中剂量锰组(44.6±4.0)％(t=3.813,P=0.019),牛磺酸+高剂量锰组(28.9±13.2)％明显低于高剂量锰组(57.7±6.2)％(t=3.709,P=0.021);电镜下观察,锰组神经元呈凋亡和坏死的形态学改变,牛磺酸+锰组与其比较有所改善.结论 锰在体外可剂量依赖性引起海马神经元死亡,牛磺酸可在一定程度上拮抗锰的神经毒性.
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| 关 键 词： | 锰 牛磺酸 海马神经元 凋亡 坏死 |
Protective effect of taurine on injury of hippocampus neurons induced by manganese in rats |
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| YAO Xue-ting
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GUO Song-chao.Protective effect of taurine on injury of hippocampus neurons induced by manganese in rats[J].Chinese Journal of Public Health,2011,27(12). |
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| Authors: | YAO Xue-ting GUO Song-chao |
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| Institution: | YAO Xue-ting,GUO Song-chao.Department Food Safety and School Health,Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control(Nanning 530028,China) |
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| Abstract: | Objective To explore protective effect of taurine on the injury of hippocampus neurons induced by manganese.Methods Primary cultures of hippocapus neurons from newly born Wistar rats were prepared.Cultured neurons were divided randomly into 8 groups of normal cultured,taurine,manganese treated(low,moderate,high),and taurine+manganese treated.The activity of lactate dehydrogenase(LDH) and apoptosis rates(a half quantitively and quantitively)were detected and the morphological changes of the neurons were obse... |
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| Keywords: | manganese taurine hippocampus apoptosis necrosis |
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