聚丙交酯-猪衍生异种骨复合材料的体外细胞亲和性研究

目的研究聚丙交酯（poly—L—lactide，PLI。A）-猪衍生异种骨（porcine—derived xenogeneic bone，PDXB）复合材料作为骨组织工程细胞支架材料的可行性。方法采用溶液浇铸法制备PLLA—PDXB复合膜，溶液浇铸一致孔剂溶出法制备PLLA—PDXB复合支架。对PLLA—PDXB复合膜和支架的表面进行水解处理，扫描电镜观察形貌特征，并测量复合膜的吸水率。用鼠OCT-1类成骨细胞作为种子细胞进行体外培养和扩增，并种植于复合膜和支架上，观察OCT-1类成骨细胞在复合膜上的黏附率、黏附形态、增殖活力和生长形态。结果PDXB粉末粒径均匀分布在50μm左右，物相结构与羟基磷灰石相似，但钙磷比降低。PLLA—PDXB复合材料经表面碱水解处理，PDXB粉末得以暴露。复合材料的亲水性和粗糙度明显增加。复合材料的细胞黏附率及细胞增殖活力均优于纯PLLA材料；细胞倾向于生长在有PDXB粉末暴露的表面上；在复合支架上培养的细胞能够迁移至支架内部旺盛生长。结论PLLA—PDXB复合材料具有优良的细胞亲和性，可望成为一类新的骨组织工程细胞支架材料。

RESEARCH ON CELL AFFINITY OF POLY-L-LACTIDE/PORCINE-DERIVED XENOGENEIC BONE COMPOSITE IN VITRO

OBJECTIVE: To evaluate the feasibility of poly-L-lactide(PLLA)/porcine-derived xenogeneic bone (PDXB) composite as a scaffold for the bone tissue engineering. METHODS: The film and the scaffold of the PLLA-PDXB composite were respectively prepared by a solution casting method and a solution casting-particle leaching method. The composite film and scaffold were further treated by the surface alkaline hydrolysis. The surface morphology of the composite was observed by the scanning electron microscopy, and hydrophilicity degree of the composite was measured. The OCT-1 osteoblast-like cells were cultured and amplified in vitro as the seeding cells, which were then implanted on the film and scaffold. The adherence rate, adherence shape,proliferating activity, and growing morphology of the OCT-1 osteoblast-like cells were observed on the film. RESULTS: The PDXB particle 50 microm in diameter on average had a similar phase structure to that of hydroxyapatite. But its Ca/P ratio was lower than that of hydroxyapatite. After the surface alkaline hydrolysis, the PDXB particle could be exposed on the surface of the PLLA-PDXB composite. The surface roughness and hydrophilicity of the PLLA-PDXB composite were obviously enhanced. The cell adherence rate and the cell proliferation activity of the PLLA-PDXB composite were higher than those of the pure PLLA material. The cells tended to grow on the exposed surface of the PDXB particles. The cells seeded on the composite scaffold could migrate to the inside of the composite scaffold and grew well. CONCLUSION: The PLLA-PDXB composite has a good cell affinity, and this kind of composite can hopefully become a new scaffold material to be used in the bone tissue engineering.