无细胞骨胶原基质的理化性能和组织相容性研究

制备了一种无细胞骨胶原基质(Acellular bone collagen matrix,ABCM)和ABCM-PDLLA复合材料。对材料的各种理化性能进行测试,并通过酶联免疫吸附(Enzyme linked immunosorbent assay,ELISA)和兔桡骨骨干缺损修复实验评价材料的组织相容性。结果表明材料中主要的骨矿成分和有机成分分别是碳酸盐羟基磷灰石和胶原,脂肪和细胞成分被完全脱除;ABCM材料具有较好的力学强度和微孔结构,加入PDLLA有助于提高材料的力学性能;ELISA检测显示材料引起的免疫反应持续时间较短,加入PDLLA可降低免疫反应。体内移植实验表明材料主要以传导成骨方式实现骨的修复和替代。总之,两种材料均具有较好的组织相容性,ABCM-PDLLA具有更好的力学性能和较低的免疫原性,更适合用作骨移植材料或骨组织工程支架材料。

Characterization and Histocompatibility of Acellular Bone Collagen Matrices

A processing technique has been developed to prepare acellular bone collagen matrix(ABCM)and ABCM-PDLLA composite materials.The properties of these materials were characterized through several different methods.The histocompatibility of the materials were investigated by ELISA(enzyme linked immunosorbent assay)test and healing the defection of New Zealand white rabbit bilateral radius.The spectroscopy indicated that the major inorganic and organic components of the bone blocks were carbonated hydroxyapatite and collagen respectively,and the fatty and cellular components were completely eliminated.The test results also revealed that the materials had good mechanical property and well-internnected pore structure,and the addition of PDLLA increased the strength of the materials.The ELISA results demonstrated that the materials had low immunogenicity in short order,and the degree of immune response caused by ABCM was greater than that by ABCM-PDLLA.All of the grafts exhibited good osteoconductive ability and a new bone form after the creeping substitution.In conclusion,two kinds of materials with good histocompatibility have been prepared,and owing to its good mechanical performance and low immunogenicity,ABCM-PDLLA is a better candidate for bone substitute and bone tissue engineering scaffold when compared with single ABCM.