| 反相高效液相色谱法测定大鼠血浆中大黄酸的含量 |
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| 引用本文： | 陆奇志,戴智勇.反相高效液相色谱法测定大鼠血浆中大黄酸的含量[J].中国药房,2002,13(3):143-144. |
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| 作者姓名： | 陆奇志 戴智勇 |
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| 作者单位： | 1. 广西壮族自治区江滨医院药剂科,南宁市,530021
2. 中南大学湘雅医院,长沙市,410008 |
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| 摘 要： | 目的 :建立血浆中大黄酸的反相高效液相色谱 (RP-HPLC)测定法。方法 :色谱柱为HypersilC18 柱 ,流动相为甲醇 -0 1 %磷酸溶液(pH3 0)(70∶30) ,流速为1 0ml/min ,在435nm波长处作紫外扫描检测。结果 :在此分析条件下对大黄酸的测定无干扰 ;在0 032～4 8μg/ml浓度范围内大黄酸峰面积与其浓度间呈现良好的线性关系 ,r=0 9990 ;血浆中大黄酸最低检测浓度为16ng/ml ,回收率为92 09 %～98 53 % ,日内、日间RSD分别为1 65 %～6 68 %和4 93 %～10 01 %。结论:本法样品预处理操作简便 ,提取完全 ,灵敏度高 ,可用于大黄酸生物样品分析
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| 关 键 词： | 大黄酸 血药浓度 RP-HPLC |
| 文章编号： | 1001-0408(2002)03-0143-02 |
| 修稿时间： | 2001年10月16 |
Determination of the Concentration of Rhein in Plasma by RP－ HPLC |
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| LU Qizhi.Determination of the Concentration of Rhein in Plasma by RP－ HPLC[J].China Pharmacy,2002,13(3):143-144. |
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| Authors: | LU Qizhi |
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| Abstract: | OBJECTIVE:To establish a RP-HPLC method for determining rhein in plasma.METHODS:Column:Spherisorb C18(4.0mm×250mm,5μm),Mobile phase:0.1% of phosphoric acid(pH 3.0)-methanol(30∶70,V/V),Flow rate:1.0ml/min;Detection wavelength:435nm.RESULTS:Rhein was completely separated and no interference was found.The peak areas of rhein within the range between 0.032～4.8μg/ml showed good linearity of the concentrations,r=0.9 990.The detection limit of rhein in plasma was 16ng/ml.The recovery rate was 92.09%～98.53%.The within-day RSD was 1.65%～6.68%,and between-day RSD was 4.93%～10.01%.CONCLUSION:This method is simple and sensitive,and can be used for analysing rhein-containing biological samples. |
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| Keywords: | rhein plasma concentration RP-HPLC |
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