小儿急性肠梗阻细菌移位及血清IL-10变化的临床意义

目的应用PCR技术检测小儿急性肠梗阻细菌移位,探讨血清白细胞介素-10（IL-10）变化及临床意义。方法应用聚合酶链反应（PCR）定性检测小儿急性肠梗阻各组及对照组全血细菌DNA;应用酶联免疫吸附试验（ELISA）检测小儿急性肠梗阻组术前、术后及对照组血清中IL-10浓度。结果肠切除肠吻合手术组16SrRNA、大肠杆菌（BG）阳性率分别为86.4%、72.7%;未切除肠管手术组16SrRNA、BG阳性率分别为64.3%、42.8%;保守治疗组16SrRNA、BG阳性率分别为57.1%、28.5%,正常血清对照组16SrRNA、BG均未检出;肠梗阻各组术前血清IL-10浓度高于对照组（P〈0.01）;各组间术前血清IL-10浓度比较有差异（P〈0.05）;肠梗阻各组术前与术后比较有差异（P〈0.01）。结论急性肠梗阻患儿发生细菌移位,随病情加重发生细菌移位机会增加,检测IL-10对小儿急性肠梗阻细菌移位的发生机制及判断愈后有着重要意义。

Study on clinical value of bacterial translocation and expression change of IL-10 in children with acute intestinal obstruction

Objective Polymerase chain reaction(PCR) was used to detect the bacterial translocation in children with acute intestinal obstruction and to investigate clinical value of interleukin-10(IL-10) change in serum.Methods The presense of bacterial DNA in whole blood were detected by polymerase chain reaction(PCR);Enzyme-linked immunosorbent assay(ELISA) was used to detect the levelof IL-10 in serum.Result In the bowel resection anastomosis group and without bowel resection group,16SrRNA were positive in 86.4% an...