| 粒细胞集落刺激因子对大鼠脑缺血再灌注后Bcl-2及Bax表达的影响 |
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| 引用本文： | 王超,马静萍. 粒细胞集落刺激因子对大鼠脑缺血再灌注后Bcl-2及Bax表达的影响[J]. 中国医疗前沿, 2011, 6(4): 36-37,52 |
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| 作者姓名： | 王超 马静萍 |
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| 作者单位： | 山西医科大学第一临床医学院神经内科,太原,030001 |
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| 摘 要： | 目的观察大鼠大脑中动脉缺血再灌注模型在给予药物粒细胞集落刺激因子（G-CSF）后脑组织bcl-2及bax表达的情况,探讨G-CSF的神经保护机制。方法将36只健康雄性SD大鼠按随机分组原则分为假手术组、模型＋溶媒组及药物组共3组。采用TTC法测脑梗死体积;采用TUNEL法测凋亡细胞数;采用免疫组化法测Bcl-2及Bax表达情况并用医学图像分析系统测定灰度值。结果与模型＋溶媒组相比,G-CSF治疗后脑梗死体积减少;凋亡细胞数减少;Bcl-2表达增加;Bax表达减少;差异均有统计学意义（P〈0.05）。结论 G-CSF治疗可以减少大鼠脑缺血再灌注损伤后脑细胞的凋亡,这可能是G-CSF神经保护作用的机制之一。
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| 关 键 词： | 粒细胞集落刺激因子 脑缺血再灌注 Bcl-2 Bax 细胞凋亡 |
Effects of Granulocyte-Colony Stinulating Factor on Expression of Bcl-2 and Bax in Rats with Focal Cerebral Ischemia Reperfusion |
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| WANG Chao,MA Jing-ping. Effects of Granulocyte-Colony Stinulating Factor on Expression of Bcl-2 and Bax in Rats with Focal Cerebral Ischemia Reperfusion[J]. China Healthcare Innovation, 2011, 6(4): 36-37,52 |
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| Authors: | WANG Chao MA Jing-ping |
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| Affiliation: | WANG Chao,MA Jing-ping.Department of Neurology,The First Clinical Medical College,Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective To observe the expression of Bcl-2 and bax in brain tissue of the middle cerebral artery occlusion/reperfusion(MCAO/R) model,when given Granulocyte-Colony Stinulating Factor(G-CSF) treatment.And to explore neuroprotective effect of G-CSF and its mechanism.Methods 36 adult healthy male SD rats were randomly divided into sham-operated group,vehide group and G-CSF treatment group.Infarct volume was measured by triphenyltetrazolium chloride staining.TUNEL technique was carried out to detect the change... |
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| Keywords: | Bcl-2 Bax |
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