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| VEGF_(121)反义核酸抑制裸鼠胰腺癌移植瘤的增殖及血管生成 | |
| 引用本文： | 韩松,王跃祥,宋后燕,倪泉兴,花天放.VEGF_(121)反义核酸抑制裸鼠胰腺癌移植瘤的增殖及血管生成[J].中国现代普通外科进展,2009,12(8):653-656. |
| 作者姓名： | 韩松 王跃祥 宋后燕 倪泉兴 花天放 |
| 作者单位： | 1. 上海交通大学附属上海市第一人民医院分院,外科,上海,200081 2. 复旦大学上海医学院分子遗传教研室,上海,200032 3. 复日大学上海医学院附属华山医院,普外科,上海,200040 |
| 摘 要： | 目的:探讨VEGF121反义核酸对裸鼠胰腺癌移植瘤的增殖及血管生成的影响,探索通过VEGF反义核酸抑制肿瘤生长.方法:将稳定转染有VEGF反义核酸pcDNA3-ASVEGF121的胰腺癌细胞系Bx-PC-3接种于裸鼠背部皮下.将实验鼠分为实验组、对照组和空白组.定期测定裸鼠移植瘤体积,免疫组织化学法检测裸鼠移植瘤VEGF、Flk-1表达水平.测定裸鼠移植瘤微血管密度.结果:VEGF反义核酸转染BxPC-3细胞后,裸鼠胰腺癌移植瘤生长明显减缓.移植瘤VEGF表达明显受到抑制,Flk-1表达水平上调.裸鼠胰腺癌移植瘤微血管密度明显降低.结论:人反义核酸VEGF121能显著抑制裸鼠胰腺癌移植瘤的增殖,并能抑制裸鼠胰腺癌移植瘤的血管生成. |
| 关 键 词： | 血管内皮生长因子类 寡核苷酸类 反义 胰腺肿瘤 小鼠 裸 |
Human antisense VEGF_(121) expression vector inhibits the proliferation and angiogenesis of pancreatic cancer in nude mouse |
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| HAN Song,WANG Yue-xiang,SONG Hou-yan,NI Quan-xing,HUA Tian-fang.Human antisense VEGF_(121) expression vector inhibits the proliferation and angiogenesis of pancreatic cancer in nude mouse[J].Chinese Journal of Current Advances in General Surgery,2009,12(8):653-656. | |
| Authors: | HAN Song WANG Yue-xiang SONG Hou-yan NI Quan-xing HUA Tian-fang |
| Institution: | Shanghai 200081;China;Shanghai 200032;China;Shanghai 200040;China |
| Abstract: | Objective: To explore the effect of antisense VEGF121 on proliferation and angio-genesis of transplant pancreatic tumor in nude mouse, so as to depress the growth of tumor by antisense VEGF121. Methods: Pancreatic cancer cell BxPC-3 transfected with pcDNA3-ASVEGF121 was injected subcutaneously into nude mouse to establish a successful transplant model of pancreatic cancer. Volume changes of the transplants were observed. VEGF and Flk-1 expression were tested by immunohistochemical stain. Transplants microve... |
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