改良扩张方法对兔静脉移植物内皮细胞保护作用研究

目的:观察不同扩张方法对兔静脉移植物内皮细胞保护作用。方法:健康纯种新西兰兔72只,按扩张液(空白对照、罂粟碱和罂粟碱+维拉帕米)随机分为A组、B组、C组,每组24只。每组又分为4个亚组,用相同扩张液分别以30mmHg、40mmHg、50mmHg和100mmHg扩张2min,再保存30min。实验终了取静脉,测量扩张后单位面积血管内皮细胞死亡数目,测定内皮细胞功能。结果:相同扩张压力下,扩张后各组均有内皮细胞死亡,但C组明显低于A组和B组(P<0.01);各组内皮细胞功能均有降低,C组明显高于A组、B组(P<0.01)。A组和B组扩张压力在50mmHg时,内皮细胞损伤最轻;而C组以40mmHg最佳(P<0.01)。结论:新的扩张方法可减轻兔静脉移植物内皮细胞损伤。

Protective effect of improved distension on endothelial cell of vein graft

Objective:To evaluate the effects of improved distension on endothelial cell of vein graft.Method:72 rabbits were divided into 3 groups randomly(group A,B,C)to observe different distension solution(control、Papaverine、Papaverine+Verapamil).In each group,the rabbits were separated into 4 sub-groups undergoing different distension pressures(30,40,50,100 mmHg).All harvested external jugular veins were distended by different pressure and different distension solution respectively.The number of dead vascular endothelial cell and endothelial cell function were measured by confocal.Result:At same pressure,in group C,the number of dead vascular endothelial cell was less than other two groups(P<0.01),and endothelial cell function was much better too;In group A and B,the best distension pressurewas 50 mmHg,while in group C,it was 40 mmHg.Conclusion:Improved distension can improve the protective effect on endothelial cell of vein graft.