小鼠囊胚体外植入恶性肿瘤细胞和容受性子宫内膜上皮细胞的比较性研究

目的 在已建立的小鼠囊胚-恶性肿瘤细胞体外共培养的基础上，将小鼠囊胚体外植入恶性肿瘤细胞的生物学行为和小鼠囊胚植入单层容受性子宫内膜上皮细胞的生物学行为进行比较性研究。方法 采用体外小鼠囊胚-恶性肿瘤细胞共培养体系和改进的小鼠囊胚-单层容受性子宫内膜上皮细胞共培养体系，光镜下观察小鼠囊胚在单层恶性肿瘤细胞和容受性子宫内膜上皮细胞贴壁、脱带、粘附及植入过程，HE染色，扫描电镜观察和免疫组织化学对两系统中囊胚植入的生物学行为进行比较性研究。结果 小鼠囊胚在恶性肿瘤细胞Hepal-6、CHO和B16共培养系统中的脱带率、贴附率和扩展率分别是69．74％、81．49％、90．15％；68．63％、79．86％、90．32％和68．94％、80．21％、89．53％。容受性子宫内膜上皮细胞共培养系统中的脱带率、贴附率和扩展率分别是69．52％、81．25％和91．51％，小鼠囊胚在恶性肿瘤细胞与容受性子宫内膜上皮细胞体外植入比较，其脱带率、贴附率和扩展率差异无统计学意义（P〉0．05），植入的形态学和滋养层细胞表达的MMP-9差异无统计学意义（P〉0．05）。结论 在体外贴壁细胞的共培养系统中，小鼠囊胚在恶性肿瘤细胞和容受性子宫内膜上皮细胞共培养系统中的发育和植入行为差异无统计学意义，可能是早期生命在体外高适应和自我组织能力的一种体现。

Comparative Research on the Implantation of Mouse Blastocyst in the Co-cultural System of Malignant Tumor Cells and Receptive Endometrium in vitro

Objective Comparaticely to study biological behaviour variability of mouse embryo implantation in vitro between malignant tumor cells and receptive endometrium, on the basis of the co-culture of mouse blastocyst and malignant tumor cells in vitro. Methods The light scope, scanning electron scope, the HE and immunohistochemical staining were applied to investigate and observe the blastocyst biology behavior in co-cultural system in vitro of mouse blastocyst-malignant cell and blastocyst-monolayer receptive endometrium. Results There was no significant biological behaviors observed or discovered for mouse blastocysts to implant into co-cultural system with malignant tumor cells from different histologcial origins, and no significant rates obtained for hatchment, attachment and outgrowth of mouse blastocysts in the co-culture system(P>0.05) of monolayer receptival endometrium cells and malignant tumor cells. There was no significant difference observed between the cell morphology changes of implanted in endometrium and MMP-9 expressed in trophoblastic cells. Conclusions That the mouse blastocyst cells has no biological behaviour difference happened between two co-cultural systems in vitro indicates it may be relevant with its high adaptation to environment and self assembly of the early life.