流通式化学发光维生素B1传感器

维生素Ｂ1亦称硫胺 ,广泛存在于豆类、谷物、酵母和肉类中 ,对维持人体的正常代谢有重要意义。已报道的维生素Ｂ1的分析方法有微生物法和化学法[1] ,美国药典 2 1版将维生素Ｂ1氧化为硫胺荧的荧光法[2 ] 已被纳入药物分析的标准方法。　　维生素Ｂ1在碱性介质中可被氧化剂氧化 ,生成硫胺荧和二硫化硫胺 ,前者是一种荧光体 ,也是一种化学发光体 ,由此而建立的维生素Ｂ1化学发光分析方法已有报道[2 ] ,而二硫化硫胺则不呈现任何光发射。选择适当的条件可以使以硫胺荧反应为主 ,在一个流动注射系统中 ,只要化学发光条件和反应条件达成和谐状态 …

A CHEMILUMINESCENCE FLOW SENSOR FOR VITAMIN B1

AIM: To determine vitamin B1 in pharmaceutical preparations by chemiluminescence (CL) flow sensor. METHODS: When 200 microL of Na3PO4 was passed through an anion exchange column, K3Fe(CN)6 was eluted from the resin and then mixed with the vitamin B1 stream containing NaOH to product CL, by the fast oxidation reaction between vitamin B1 and K3Fe(CN)6 in alkaline solution. RESULTS: The CL emission intensity was correlated with the vitamin B1 concentration in the range 1.0 x 10(-5) to 1.0 x 10(-3) mol.L-1 and the detection limit was 8.0 x 10(-6) mol.L-1 (3 sigma). A complete analysis, including sampling and washing, can be performed within 2 min with a relative standard deviation of less than 4.0%. CONCLUSION: The vitamin B1 flow sensor was stable for over 200 analyses and has been applied successfully to determination of vitamin B1 in pharmaceutical preparations.