博安霉素对小鼠结肠癌及其肝转移的抑制作用

目的　研究博安霉素对小鼠结肠癌及肝转移的疗效。方法　用小鼠结肠癌26移植于皮下、盲肠、肝内及脾内移植肝转移等模型,并应用LEICAQ500IW图象分析系统分析肝转移病理切片。结果　博安霉素5mg·kg^-1和2.5mg·kg^-1，对皮下瘤抑瘤率为78.7％和61.9%;盲肠移植瘤抑瘤率为99.4％和90.0%;肝内移植瘤的抑瘤率为86.9％和75.7%。博安霉素10mg·kg^-1和5mg·kg^-1对脾内原发灶抑瘤率为98.1％,99.4%和52.6%,76.2%,对肝转移灶的抑瘤率为93.1％,97.6%和54.5%,56.8%。结论　博安霉素明显抑制小鼠结肠癌26在皮下、盲肠、肝内和脾内移植瘤的生长，对肝转移也有显著抑制作用。

INHIBITORY EFFECT OF BOANMYCIN ON THE GROWTH OF COLON CARCINOMA 26 AND HEPATIC METASTASIS IN MICE

AIM: To investigate the therapeutic effect of boanmycin (BAM, bleomycin A6) on colon carcinoma 26 and its hepatic metastasis in mice. METHODS: A series of models including subcutaneous transplantation, orthotopic transplantation in cecum subserosa, intra-hepatic transplantation of tumor, and intrasplenic transplantation of tumor accompanied with hepatic metastases were employed. In addition, LEICA Q 500IW image analysis system was used to determine the area of metastatic lesions in the liver in histopathological sections. RESULTS: BAM at 5 mg.kg-1 and 2.5 mg.kg-1 inhibited the growth of subcutaneous tumors by 78.7% and 61.9%, and the orthotopic tumors by 99.4% and 90.0%; and the intra-hepatic tumors by 86.9% and 75.7%, respectively. Determined by the numbers of metastatic nodules, BAM at 10 mg.kg-1 and 5 mg.kg-1 inhibited hepatic metastases from intra-splenic transplantation by 97.6% and 56.8%; and evaluated by image analysis of metastatic lesions, by 100% and 63.3%, respectively. CONCLUSION: Boanmycin is highly effective in a panel of models including the subcutaneous, orthotopic, intra-hepatic transplanted tumors, and hepatic metastases of murine colon carcinoma 26.