力达霉素对人结肠癌HCT-8细胞侵袭调节基因表达的影响

目的研究抗肿瘤抗生素力达霉素对人结肠癌HCT-8细胞侵袭调节基因表达的影响。方法利用cDNA排列(cDNAarrays)、Northern杂交、RT-PCR技术。结果力达霉素可以促进TIMP-1的表达，抑制MMP-9的表达，cDNA排列、Northern杂交、RT-PCR检测得到了同样的结果。结论力达霉素可能通过抑制IV型胶原酶的产生，同时诱导金属蛋白酶抑制因子的产生，从而表现出抗侵袭活性。

EFFECT OF LIDAMYCIN ON THE EXPRESSION OF GENES INVOLVED IN INVASION REGULATION IN HCT-8 HUMAN COLON CANCER CELLS

AIM: To study the effect of lidamycin on the expression of genes involved in invasion regulation in HCT-8 human colon cancer cells. METHODS: HCT-8 human colon cancer cells were treated with lidamycin (10 nmol.L-1) for 8 h. The effect of lidamycin on the expression of genes were detected by cDNA arrays. Northern blot and RT-PCR. RESULTS: Hybridization of the entire cDNA populations to Atlas Arrays showed that lidamycin down-regulated the expression level of MMP-9 and up-regulated the expression level of TIMP-1. These changes were confirmed by Northern blot and RT-PCR. CONCLUSION: The results indicate that lidamycin may exhibit its anti-invasive activity by inhibitting the production of type IV collagenase whilst enhancing the production of tissue inhibitor metalloproteinase.