普罗布考对兔血管成形术后再狭窄及血管生长因子的影响

目的 :研究普罗布考 (Probucol)对兔血管成形术后再狭窄的预防作用和对生长因子表达的影响。方法 :雄性新西兰白兔2 0只 ,随机分为对照组 (C组 ) 1 0只 ,Probucol组 (P组 ) 1 0只 ,于高脂饮食 1周后行髂动脉球囊拉伤术 ,术后 C组单纯予高脂饮食喂养 ,P组予 Probucol每只 1 0 0 0 m g/ d喂养 ,各组均给予 4周药物。于实验第 1、8、2 1、35天行血脂检查 ,术后 4周行血管形态学检查及检测胰岛素样生长因子 - I受体 (IGF- IR)、血管内皮生长因子 (VEGF)、增殖细胞核抗原 (PCNA)。结果 :两组间各时点血脂浓度差异无统计学意义。与 C组相比 ,P组管腔面积明显扩大 (P <0 .0 1 ) ,内膜 +中膜面积明显减少 (P <0 .0 5 ) ,IGF- IR、VEGF和 PCNA表达明显减少 (P <0 .0 1 )。结论 :Probucol可以显著减轻兔血管成形术后再狭窄的形成 ,抑制 IGF-IR、VEGF和 PCNA的表达

EFFECT OF PROBUCOL ON THE PREVENTING RESTENOSIS AND THE EXPRESSION OF GROWTH FACTORS AFTER BALLOON ANGIOPLASTY IN RABBITS

Objective:To investigate the effect of probucol on preventing restenosis and the expression of growth factors after balloon angioplasty in rabbits. Methods:20 male New Zealand white rabbits were randomly divided into control group(group C,n=10)and probucol group (group P,n=10) and were injured by balloon on iliac artery after one week of hypercholesterolaemic diet. After the injury,the rabbits of Group C were only fed with hypercholesterolaemic diet,and those of Group P were given probucol 1 000 mg/d.Both groups were given drugs for 4 weeks.At the 1st, 8th, 21st and 35th day, the plasma concentrations of lipids were examined in all rabbits. At the end of 4 weeks after injury, the iliac arteries were examined with histomorphometrical method, examined Insulin like Growth Factor I Receptor(IGF IR), Vascular Endothelial Growth Factor (VEGF) and Proliferating Cell Nuclear Antigen (PCNA) .Result:The plasma concentrations of lipids had no significant difference between two groups.Compared with that of group C, the lumen area, the intimal and medial area were larger in Group P. The expression of IGF IR, VEGF and PCNA in Group P were decreased significantly.Conclusion:Probucol can prevent the restenosis of rabbit's iliac artery after balloon injury, decreasing the expression of IGF IR, VEGF and PCNA.