EIAV减毒疫苗诱导的特异性细胞免疫应答

目的：研究马传染性贫血病毒（EIAV）减毒疫苗株接种动物体内诱导的细胞免疫学反应方法：选用健康没有EIAV感染的马，接种EIAV减毒疫苗株DLV，运用流式细胞术检测疫苗株诱导的马外周血单核细胞（PBMC）中CD4和CD8阳性淋巴细胞数量的变化，以及^3H测定特异性淋巴细胞增殖反应和^51Cr释放实验测定淋巴细胞杀伤反应：结果：EIAV减毒疫苗株DLV免疫动物后，能够引起CD8^＋细胞明显增加．免疫马PBMC在EIAV刺激下．引起EIAV特异性淋巴细胞增殖反应（SI≥3），并能检测出EIAV特异性CTL活性，靶细胞杀伤百分率高于30％：结论：EIAV减毒疫苗株DLV免疫动物能够引起特异性EIAV细胞免疫反应，极有可能参与宿主的保护免疫作用。

Induction of EIAV-specific cellular immune response by attenuated EIAV vaccine

AIM: To elucidate cellular immune protective mechanism of EIAV. METHODS: Four horses were immunized with (DLV) by subcutaneous injection and 2 horses with 0.85% sodium chloride as the negative control. Rectal temperatures and clinical features were recorded daily. Whole blood samples were collected, from which PBMC were separated and used for CTL assay and lymphocyte proliferation assay. RESULTS: The target cells were activated by PWM and treated with DLV or recombinant vaccinia vectors expressing DLV and LN Gag or Env separately. The percentage of EIAV-specific CTL lysis was under 5% in the negative control group, however, that was 20% to 30% in the group of immunized horses. The stimulation index (SI) from proliferation assay was 3 in the group immunized with attenuated DLV vaccine. CONCLUSION: The results indicated that EIAV-specific cellular immune-response induced by DLV immunization may contribute to the immune protective effect against EIAV virus infection.