针刺对备用背根节神经元NP-1表达的影响

目的探讨针刺对备用背根节（dorsal root ganglion，DRG）NP-1表达的影响。方法将25只成年雄猫分为5组，即假手术组、单侧备用背根手术7d组、单侧备用背根手术并针刺7d组、单侧备用背根手术14d组和单侧备用背根手术并针刺14d组。通过免疫组化ABC法，观察各组备用DRG中NP-1的免疫反应神经元数目。结果在假手术组，NP-1阳性神经元主要定位于部分中小神经元及少数大神经元胞浆中；部分背根切断7d后NP-1阳性中小神经元数较假手术组明显减少（P〈0．05），NP-1阳性大神经元数与假手术组者差异无统计学意义（P〉0．05）；而术后14d，NP-1阳性中小神经元数较7d组明显增多（P〈0．05），且回升至假手术组水平，NP-1阳性大神经元数与手术7d组差异无统计学意义（P〉0．05）；针刺7d组较手术7d组NP-1阳性中小神经元数增多（P〈0．05），但尚未回升至假手术组水平，NP—1阳性大神经元数减少（P〈0．05）；针刺14d组各类阳性神经元数目与手术14d组及针刺7d组差异均无统计学意义（P〉0．05）。结论针刺可上调NP-1在备用DRG中小神经元的表达。

Impact of Acupuncture on NP-1 Expression in Spared Dorsal Root Ganglion

OBJECTIVE: To investigate the impact of acupuncture on NP-1 expression in spared dorsal root ganglion (DRG). METHODS: Twenty-five male adult cats were divided into five groups. The five cats in the pseudo-operation group were kept intact. Ten cats were subjected to unilateral root rhizotomy(unilateral L1-L5 DRGs were transected, but L6 DRG was spared), with five sacrificed seven days later and another five sacrificed 14 days later. Ten cats with unilateral root rhizotomy received acupuncture (electron-needle stimulation in Zusanli, Xuanzhong, Futu and Sanyinjiao which are located in the distribution area of L6 spinal nerve) for 30 minutes per day. Five cats in the acupuncture group were sacrificed seven days and 14 days later respectively. The L6 DRG from the experimental side of each animal was taken and made into frozen sections 20 microm in thickness. The sections were stained under the same conditions using NP-1 specific antibody (1:600, Santa) by immunohistochemistry ABC method. The amounts of NP-1 immunoreaction neurons of DRG were measured. RESULTS: The NP-1 immunoreactants were mainly located in the small and medium-sized neurons and a few large sized neurons in the cats of the pseudo-operation group. Less NP-1 positive medium-small sized neurons were found seven days after partial dorsal root rhizotomy (P<0.05). But they returned back to the original level 14 days after operation. The NP-1 positive large neurons had no significant changes after operations. With seven days of acupuncture, the amounts of NP-1 positive small and medium-sized neurons increased significantly (P<0.05) but were still less than the original level (P<0.05). There were no differences in neurons 14 days after operations between the acupuncture and non-acupuncture group (P>0.05) and between the groups with seven days acupuncture and 14 days of acupuncture (P>0.05). CONCLUSION: Acupuncture increases the expression of NP-1 in small and medium-sized neurons of spared DRG.