神经生长因子在大鼠结直肠内脏高敏感形成中的作用

目的 探讨在大鼠结直肠内脏高敏感形成中,神经生长因子所起的作用.方法 以注射神经生长因子+假避水应激(N组,8只)和注射神经生长因子+慢性避水应激(NW组,8只)方法处理大鼠,建立内脏高敏感动物模型,以腹腔注射0.9%氯化钠溶液+假避水应激处理大鼠作为正常对照(C组,8只).造模前后检测结直肠扩张(CRD)后大鼠内脏运动反射变化程度并进行统计学分析.结果 大鼠分组处理前,CRD后内脏运动反射值(WMR值)均差异无统计学意义(P＞0.05).分组处理后,N组和NW组大鼠在CRD压力为20、40和60 mmHg(1 mmHg=0.133 kPa)时,VMR值与C组比较差异均无统计学意义(P＞0.05);而在CRD压力为80 mmHg时,NW组大鼠VMR值(3.54)与C组(2.12)比较差异有统计学意义(P=0.016);而N组大鼠在此压力下VMR值(2.62)虽较C有所升高,但差异无统计学意义(P＞0.05).结论 在应激状态下,应用神经生长因子可明显促进大鼠结直肠内脏高敏感形成.

Effect of nerve growth factor on colorectal visceral hypersensitivity in rats

Objective To study the potential role of nerve growth factor(NGF)on visceral hypersensitivity in rats.Methods Visceral hypersensitivity was induced by intraperitoneal injection of NGF with or without water avoidance stress in N group(n=8)and NW group(n=8),respectively.The rats in controI group(n=8)were intraperitoneaIly injected with normal saline and received shame water avoidance stress.Electromyogram activity of the external oblique musculature was measured by means of the visceromotor response(VMR)during colorectal distention(CRD).Statistical analysis was made by ANOVA.Results There was,nb statistical difference among three groups in the VMR at pressure of 20,40 or 60 mmHg(1 mmHg=0.133 kPa).When the pressure at 80 mmHg,the VMR was higher in NW group(3.54)than that in control group(2.12)(P=0.016).while it was 2.62 in N group with no difference to control group.Conclusion The colorectal visceral sensitivity in rats may be promoted by NGF in presence of water avoidance stress.