新城疫病毒体外抗白血病作用的实验研究

 目的　通过体外实验研究探讨新城疫病毒（NDV）对人类急性单核细胞白血病的作用。方法　应用倒置显微镜观察NDV感染后白血病细胞形态和密度的变化，AO／PI染色后观察病毒对白血病细胞的生长抑制情况；甲基偶氮唑蓝（MTT）法测定不同浓度NDV作用于白血病细胞不同时间后对细胞增殖的影响。结果　倒置显微镜下，对照组细胞形态规则、分布密集；NDV感染组细胞形态不规则，聚集、融合现象明显，分布稀疏；AO／PI染色后对照组呈均匀明亮的绿色荧光，NDV原液组呈红、绿两色荧光，以红色为主，MTT检测结果显示不同浓度NDV对SHI-1细胞的增殖均有明显抑制作用（P＜0.01），不同时间的抑制作用也有明显差异（P＜0.01）；增殖抑制作用呈现时间-浓度依赖关系。结论　NDV是一种有效的抗人类急性单核细胞白血病的病毒。

The effects of Newcastle disease virus on human leukemia in vitro

Objective To study the effects of Newcastle disease virus (NDV) on human leukemia in vitro. Methods The density and morphologic change of cells infected by NDV was observed. MTT assay was used to investigate the effect of NDV on the proliferation of the leukemia cells. Results The cells of the control were all regular and coarctate by inverted microscope. The effect of NDV were conspicuous in that the cells were irregular, sparsate, aggregate and fused in the experimental groups. MTT assay showed that the proliferation of SHI-1 cells were significantly inhibited by NDV with different concentration (P ＜0.01). The results also demonstrated that the difference within the concentration and the time were both statistically significant (P＜0.01). The inhibition of NDV was further confirmed in a time-concentration-dependent manner.Conclusion Newcastle disease virus can inhibit human acute monocytic leukemic cell proliferation in vitro.The safety of NDV is reliable.