血清肝纤维化指标水平与肝组织纤维图象分析的关系

目的 探讨血清肝纤维化指标透明质酸（ＨＡ），Ⅲ型前胶原（ＰＣⅢ）、Ⅲ型胶原（ＣⅣ）水平与肝组织纤维化程度的关系及对慢性病毒性肝炎肝纤维化的临床意义，方法 用放射免疫法测定１５１例慢性病毒性肝炎患者血清ＨＡ、ＰＣⅢ、ＣⅣ水平，所有患者均做肝活组织检查，对肝组织标本行Ｍａｓｓｏｎ法网状纤维染色，在计算机图象分析系统下对肝组织网状纤维染色标本进行纤维组织定量测定。结果 血清ＨＡ、ＰＣⅢ、ＣⅣ水平与肝组织

The relationship between the levels of serum fibrosis marks and morphometric quantitative measurement of hepatic histological fibrosis

OBJECTIVE: To study the relationship between the levels of serum fibrosis marks hyaluronic acid (HA), procollagen type III (PCIII), collagen type IV (CIV) and the degree of hepatic fibrosis, and the clinical significance of HA, PCIII, CIV in the diagnosis of hepatic fibrosis to the chronic viral hepatitis. METHODS: The concentrations of serum HA, PCIII, CIV in 151 patients with chronic viral hepatitis were measured by radioimmunoassay. Liver biopsies were performed in all of the patients at the same time. Morphometric quantitative measurements of hepatic fibrosis were performed in 4mum sections stained with masson stain using a fully automated image-analysis system. RESULTS: The levels of serum HA, PCIII, CIV were all increased steadily with the progression of hepatic fibrosis, and the highest levels were in stage 4. They were all positively correlated with staging of the liver pathology and morphometric measurement of hepatic fibrosis (P<0.001). The coefficient value (r) with staging were 0.694, 0.493, 0.552 (P<0.001), respectively and with morphometric measurement of hepatic fibrosis were 0.715, 0.596, 0.537 (P<0.001), respectively. CONCLUSION: The levels of serum HA, PCIII, CIV are in concert with the degree of hepatic fibrosis, and the determination of these marks may be valuable for diagnosing hepatic fibrosis.