| 纤维素与明胶微载体微重力培养肝细胞的比较 |
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| 引用本文： | 张志,周焕城,李治国,潘明新,王忠,高毅.纤维素与明胶微载体微重力培养肝细胞的比较[J].南方医科大学学报,2010,30(4):704. |
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| 作者姓名： | 张志 周焕城 李治国 潘明新 王忠 高毅 |
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| 作者单位： | 南方医科大学珠江医院肝胆二科,广东,广州,510282 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，广东省科技计划项目 |
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| 摘 要： | 目的 探讨商品化的多孔微载体:cytopore 2和cultispher S何种更适合于微重力条件下培养CL-1细胞.方法 采用RCCS旋转培养系统.在50 ml水平微重力旋转培养CL-1细胞.分别对接种至cytopore 2和cultispher S的细胞进行动态形态学观察、细胞计数和功能学检测.结果 CL-1细胞在两种载体上均可粘附生长,并于第9天达到生长高峰.细胞计数结果提示cultispher S的细胞密度可达到4×10 6个/ml.在第10、11、12天,cytopore 2组的上清中自蛋白浓度明显高于cultispher S组.有显著差异(P<0.05),在第10、11天,cytopore 2组的上清中尿素浓度明显高于cultispher S组,有显著差异(P<0.05).结论 Cytopore 2培养的CL-1细胞计数低于Cultispher S组,但功能好于Cultispher S组.
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| 关 键 词： | 微重力培养 多孔微载体 人肝细胞 人 |
Microgravity culture of hepatocytes on cellulose/gelatin macroporous microcarrier |
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| ZHANG Zhi,ZHOU Huan-cheng,LI Zhi-guo,PAN Ming-xin,WANG Zhong,GAO Yi.Microgravity culture of hepatocytes on cellulose/gelatin macroporous microcarrier[J].Journal of Southern Medical University,2010,30(4):704. |
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| Authors: | ZHANG Zhi ZHOU Huan-cheng LI Zhi-guo PAN Ming-xin WANG Zhong GAO Yi |
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| Institution: | ZHANG Zhi,ZHOU Huan-cheng,LI Zhi-guo,PAN Ming-xin,WANG Zhong,GAO Yi Department of Hepatobiliary Surgery,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China |
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| Abstract: | Objective To compare macroporous microcarrier Cytopore 2 and Cultispher S for their effects in microgravity culture of CL-1 cells.Methods CL-1 cells were cultured on Cytopore 2 and Cultispher S respectively in a rotational cell culture system (RCCS) in a volume of 50 ml.Dynamic morphological observation and cell counting and functional test were carried out during the cell culture.Results The cells were capable of adhering to and proliferating on both of the microcarriers,reaching the growth peak on day 9 o... |
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| Keywords: | microgravity culture macroporous microcarrier human hepatocytes |
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