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| 斑马鱼髓系造血细胞缺陷突变体的大规模遗传筛选 | |
| 引用本文： | 戴朝霞,颜广,陈英华,刘伟,霍中军,温宗华,刘靖,王鹍,黄志斌,马宁,陈小辉,马品芸,罗伟豪,赵颖,樊淑,黄红辉,温子龙,张文清.斑马鱼髓系造血细胞缺陷突变体的大规模遗传筛选[J].南方医科大学学报,2010,30(6). |
| 作者姓名： | 戴朝霞 颜广 陈英华 刘伟 霍中军 温宗华 刘靖 王鹍 黄志斌 马宁 陈小辉 马品芸 罗伟豪 赵颖 樊淑 黄红辉 温子龙 张文清 |
| 作者单位： | 1. 南方医科大学基础医学院,细胞生物学教研室,广东,广州,510515 2. 南方医科大学基础医学院,组织胚胎学教研室,广东,广州,510515 3. 南方医科大学基础医学院,病理学教研室,广东,广州,510515 4. 南方医科大学基础医学院,医学遗传学教研室,广东,广州,510515 5. 香港科技大学生化系,香港 |
| 基金项目： | 国家自然科学基金海外及港澳学者合作研究基金 |
| 摘 要： | 目的 通过大规模化学遗传学方法筛选斑马鱼髓系造血缺陷突变体.方法 利用化学诱变剂乙基亚硝基脲(ENU)将雄鱼精原母细胞诱变,通过与AB野生型雌性斑马鱼交配产生F1代,源自不同founder(FO)的F1代同胞之间交配产生F2家族,F3代来F2家族同胞内交配,并分别以中性红和苏丹黑B染色筛选巨噬细胞和粒细胞缺陷突变体.结果 我们筛选了350个F2家族,共1424对鱼.初步筛选到6个斑马鱼髓系造血系统的突变体,其中3个突变体为中性红染色异常,另3个突变体为苏丹黑染色异常,表明以上突变体可能存在巨噬细胞或粒细胞的发育障碍.结论 ENU化学诱变和中性红及苏丹黑B染色筛选斑马鱼髓系造血缺陷突变体是简单、价廉、有效的化学遗传学方法.通过保留突变体对后代进行进一步的研究分析,有望鉴定和克隆影响斑马鱼髓系造血新的基因或已知基因的新的调控途径. |
| 关 键 词： | 斑马鱼 髓系造血 正向遗传学筛选 苏丹黑 中性红 |
Forward genetic screening for zebrafish mutants defective in myelopoiesis |
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| DAI Zhao-xia,YAN Guang,CHEN Ying-hua,LIU Wei,HUO Zhong-jun,WEN Zong-hua,LIU Jing,WANG Kun,HUANG Zhi-bin,MA Ning,CHEN Xiao-hui,MA Ping-yun,LUO Wei-hao,ZHAO Ying,FAN Shu,HUANG Hong-hui,WEN Zi-long,ZHANG Wen-qing.Forward genetic screening for zebrafish mutants defective in myelopoiesis[J].Journal of Southern Medical University,2010,30(6). | |
| Authors: | DAI Zhao-xia YAN Guang CHEN Ying-hua LIU Wei HUO Zhong-jun WEN Zong-hua LIU Jing WANG Kun HUANG Zhi-bin MA Ning CHEN Xiao-hui MA Ping-yun LUO Wei-hao ZHAO Ying FAN Shu HUANG Hong-hui WEN Zi-long ZHANG Wen-qing |
| Institution: | DAI Zhao-xia1,YAN Guang1,CHEN Ying-hua3,LIU Wei1,HUO Zhong-jun1,WEN Zong-hua2,LIU Jing1,WANG Kun1,HUANG Zhi-bing1,MA Ning1,CHEN Xiao-hui1,MA Ping-yun4,LUO Wei-hao4,ZHAO Ying4,FAN Shu4,HUANG Hong-hui4,WEN Zi-long5,ZHANG Wen-qing1 1Department of Cell Biology,2Department of Histology & Embryology,3Department of Pathology,4Department of Medical Genetics,Southern Medical University,Guangzhou 510515,China,5Department of Biochemistry,Hong Kong University of Science and Technology,Hong Kong |
| Abstract: | Objective To identify zebrafish mutants with myelopoiesis defects by ENU mutagenesis and large-scale forward genetic screening.Methods Male zebrafish were mutagenized with N-ethyl N-nitrosourea to induce mutations in the spermatogonial cells to generate the founders,which were outcrossed with AB to raise F1 fish.The F1 fish from different founders were mated to generate the F2 families.The F3 embryos from F2 sibling crosses were screened by Sudan black B staining and neutral red staining.Results A total of ... |
| Keywords: | zebrafish myelopoiesis forward genetic screen sudan black B neutral red |
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