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| 氧化型辅酶Ⅰ抗辐射损伤作用及其量效关系的初步实验 | |
| 引用本文： | 李民英,雷风,尤玮,谭宇静,陆小军,陈龙华,张积仁. 氧化型辅酶Ⅰ抗辐射损伤作用及其量效关系的初步实验[J]. 南方医科大学学报, 2010, 30(8) |
| 作者姓名： | 李民英 雷风 尤玮 谭宇静 陆小军 陈龙华 张积仁 |
| 作者单位： | 1. 南方医科大学南方医院放疗科,广东,广州,510515;中山市人民医院放射治疗科,广东,中山,528403 2. 中山市人民医院放射治疗科,广东,中山,528403 3. 南方医科大学珠江医院肿瘤中心,广东,广州,510282 4. 南方医科大学南方医院放疗科,广东,广州,510515 |
| 摘 要： | 目的 探讨氧化型辅酶Ⅰ(NAD+)抗辐射损伤作用及其量效关系.方法 L02人正常肝细胞株加入含10%胎牛血清的RPMI 1640培养基中常规方法培养,X线照射后和在照射后即刻加入NAD+,采用MTT法检测细胞活性,TUNEL法检测细胞凋亡,观察X线照射对L02人正常肝细胞的辐射损伤,并观察NAD+对受照射L02肝细胞辐射损伤的影响作用及其与NAD+浓度的关系.结果 L02人正常肝细胞受X线照射后细胞损伤随照射吸收剂量的增大而加重,受照射后24 h细胞抑制作用最显著,细胞损伤多表现为细胞凋亡;加入NAD+可提高受照射细胞存活率,其作用在NAD+终浓度为100～1000μg/ml范围内与浓度呈正相关,在浓度大于1000 μg/ml后,加大浓度并不再显著增加受照射细胞的存活率.结论 NAD+具有一定的抗细胞辐射损伤作用,其作用在一定范围内与其浓度呈正相关关系. |
| 关 键 词： | 肝细胞 辐射损伤 凋亡 烟酰胺腺嘌呤二核苷酸 |
Effect of NAD+ against radiation injury and its dose-effect relationship |
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| LI Min-ying,LEI Feng,YOU Wei,TAN Yu-jing,LU Xiao-jun,CHEN Long-hua,ZHANG Ji-ren. Effect of NAD+ against radiation injury and its dose-effect relationship[J]. Journal of Southern Medical University, 2010, 30(8) | |
| Authors: | LI Min-ying LEI Feng YOU Wei TAN Yu-jing LU Xiao-jun CHEN Long-hua ZHANG Ji-ren |
| Affiliation: | LI Min-ying1,2,LEI Feng2,YOU Wei3,TAN Yu-jing3,LU Xiao-jun2,CHEN Long-hua1,ZHANG Ji-ren3 1Department of Radiotherapy,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China,2Department of Radiotherapy,Zhongshan People's Hospital,Zhongshan 528403,3Department of Oncology,Zhujiang Hospital,Guangzhou 510282 |
| Abstract: | Objective To explore the effect of NAD+ against radiation injury and its dose-effect relationship. Methods L02 liver cells cultured in RPMI 1640 medium containing 10% fetal calf serum were exposed to X-ray irradiation followed by immediate application of NAD+. The cellular viability was analyzed by MTT assay and the apoptotic cells were detected by TUNEL methods to observe the damages of L02 liver cells induced by X-ray exposure and analyze the dose-effect relationship of NAD+. Results The viability of L02 ... |
| Keywords: | liver cells radiation damage apoptosis nicotinamide adenine dinucleotide |
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