免疫组织化学标记在肝内胆管上皮内瘤变及癌变诊断中的应用

目的 探讨判断肝内胆管上皮内瘤变及癌变的免疫组织化学单克隆抗体标记最佳组合方案.方法 采用免疫组织化学SP法检测高级别肝内胆管上皮内瘤变及癌变组织中CK7、CK20、Villin、CEA、P53及Ki-67抗原,运用SPSS10.0软件对有关数据进行统计分析.结果 Villin在肝内胆管上皮内瘤变及癌变组织中100%表达,而在癌旁正常胆管上皮内无一例表达;CEA在高级别肝内胆管上皮内瘤变组织中的表达明显低于癌变组织(P<0.05);Ki-67在绝大部分高级别胆管上皮内瘤变中的表达指数低于癌变组织(P＜0.01);P53在高度上皮内瘤变组织中表达明显低于癌变组织(P<0.01).结论 在肝内胆管上皮组织中联合检测CEA、Villin、Ki-67及P53等多种抗原,对判断肝内胆管上皮内瘤变是否癌变具有较明确的指导意义.

Application of immunohistochemical labeling in the diagnosis of hepatobiliary intraepithelial neoplasia and/or cancer

Objective To investigate the best combination of monoclonal antibodies for the diagnosis of hepatobiliary intraepithelial neoplasia and/or cancer. Methods CK7, CK20, Villin, CEA, P53 and Ki-67 antigens were detected in the tissues of high-level hepatobiliary intraepithelial neoplasia and cancer by immunohistochemistry and the results were analyzed statistically. Results Villin was 100% positive in hepatobiliary intraepithelial neoplasia and/or cancer, while 100% negative in the adjacent normal bile duct epi...