| 蛋白激酶D3对前列腺癌细胞中基质金属蛋白酶7的负调控作用 |
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| 引用本文： | 邹志鹏,冯丽,许万福,柯志勇,Wang Q.Jane,邓凡.蛋白激酶D3对前列腺癌细胞中基质金属蛋白酶7的负调控作用[J].南方医科大学学报,2010,30(8). |
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| 作者姓名： | 邹志鹏 冯丽 许万福 柯志勇 Wang Q.Jane 邓凡 |
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| 作者单位： | 1. 南方医科大学细胞生物学教研室,广东,广州,510515
2. Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine,Pittsburgh,Pennsylvania,15260,USA |
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| 基金项目： | 国家自然科学基金，教育部留学回国人员科研启动基金 |
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| 摘 要： | 目的 探讨蛋白激酶D3(PKD3)对前列腺癌细胞中基质金属蛋白酶7(MMP-7)的调控作用.方法 应用佛波脂(PMA,100 nmol/L)处理前列腺癌PC3细胞,Western blotting和实时RT-PCR分别检测PKD3的激酶活性和MMP-7mRNA表达;将siRNA-PKD3瞬时转染PC3细胞以敲低PKD3的表达,同样用实时RT-PCR检测MMP-7 mRNA的表达状况;在HEK293细胞中,分别过表达GFP-PKD3、siRNA-PKD3敲低PKD3的表达或先过表达GFP-PKD3再siRNA-PKD3敲低PKD3的表达,以上述同样的方法比较MMP-7mRNA相对表达量的变化.结果 在PC-3细胞中,PMA诱导的PKD3的激活可明显降低MMP-7 mRNA表达,反之,应用siRNA敲低PKD3的表达则明显提高MMP-7mRNA表达水平(P<0.01);同样,在HEK293细胞中,过表达PKD3可明显降低MMP-7的表达水平,而敲低PKD3可明显提高MMP-7的表达,且敲低PKD3可逆转PKD3过表达诱导的MMP-7下调.结论 PKD3可能通过负调控MMP-7的表达而介导前列腺癌的恶性进程.
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| 关 键 词： | 蛋白激酶D3 基质金属蛋白酶7 前列腺癌 负调控 |
Protein kinase D3 is involved in negative regulation of MMP-7 in prostate cancer cells |
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| Zou Zhi-peng,FENG Li,XU Wan-fu,KE Zhi-yong,Wang Q.Jane,DENG Fan.Protein kinase D3 is involved in negative regulation of MMP-7 in prostate cancer cells[J].Journal of Southern Medical University,2010,30(8). |
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| Authors: | Zou Zhi-peng FENG Li XU Wan-fu KE Zhi-yong Wang QJane DENG Fan |
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| Institution: | ZOU Zhi-peng 1,FENG Li 1,XU Wan-fu 1,KE Zhi-yong 1,WANG Q. Jane 2,DENG Fan 1 1 Department of Cell Biology,Southern Medical University,Guangzhou 510515,China,2 Department of Pharmacology and Chemical Biology,University of Pittsburgh School of Medicine,Pittsburgh,Pennsylvania,15260,USA |
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| Abstract: | Objective To explore the role of protein kinase D3 (PKD3) in the regulation of matrix metalloprteinases 7 (MMP-7) expression in prostate cancer cells. Methods PC-3 cells were either stimulated with 100 nmol/L PMA to activate PKD3 kinase activity, or transiently transfected with PKD3 siRNA, and the relative expression level of MMP-7 mRNA were analyzed by real-time PCR using 2 method. MMP-7 mRNA levels were also analyzed and quantified in HEK293 cells with over-expression of wild-type PKD3, PKD3 knockdown (us... |
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| Keywords: | protein kinase D3 matrix metalloprteinases 7 prostate neoplasm |
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