| 小油桐叶中总多酚的含量测定 |
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| 引用本文： | 龚文菲,汪铁山,林敬明.小油桐叶中总多酚的含量测定[J].南方医科大学学报,2010,30(6). |
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| 作者姓名： | 龚文菲 汪铁山 林敬明 |
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| 作者单位： | 南方医科大学珠江医院药剂科,广东,广州,510282 |
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| 基金项目： | 广东省中医药管理局基金 |
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| 摘 要： | 目的 建立小油桐叶中总多酚的含量测定方法.方法 以没食子酸为对照品,采用Folin-Ciocalteu比色法最适宜条件下对小油桐叶中总多酚含量进行测定.结果 没食子酸对照品在浓度为0.002～0.010 g·L-1范围内与吸光度成良好的线性关系;小油桐叶片中总多酚含量为6.74%,RSD为0.75%;样品溶液在10～90 min内基本稳定(RSD=0.28%);精密度试验RSD为0.23%:重复性实验RSD为1.01%;平均回收率为99.85%.结论 该法灵敏度高,重复性好,简便快捷.
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| 关 键 词： | 小油桐 多酚 含量测定 |
Content analysis of total polyphenol in the leaves of Jatrophy curcas.L |
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| GONG Wen-fei,WANG Tie-shan,LIN Jing-ming.Content analysis of total polyphenol in the leaves of Jatrophy curcas.L[J].Journal of Southern Medical University,2010,30(6). |
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| Authors: | GONG Wen-fei WANG Tie-shan LIN Jing-ming |
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| Institution: | GONG Wen-fei,WANG Tie-shan,LIN Jing-ming Pharmacy department,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China |
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| Abstract: | Objective To establish a method for analyzing the content of total polyphenols in leaves of Jatropha curcas.L.Methods Gallic acid was used as reference substance,the content of total polyphenols was analyzed Folin-Ciocalteu chromatometry.Results There was a good linearity for gallic acid in the range of 0.002-0.010 g.L-1.The content of total polyphenols in the leaves of Jatropha curcas.L was approximately 6.74% with RSD 0.75%.The sample solution was stable during 10-90 min with RSD 0.28%.The precision RSD w... |
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| Keywords: | Jatropha curcas L polyphenol content determination |
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