人参皂甙Rg1对NOS的调控在海马神经元放射性损伤防护中的意义

目的 研究人参皂甙Rg1对原代培养大鼠海马神经元放射性损伤的保护作用及机制,为放射性脑损伤的预防提供理论依据及新的方法.方法 30Gy的X射线单次照射培养至12d的海马神经元,用DAPI染核方法检测海马神经元凋亡情况,用NOS测定试剂盒测定细胞培养液NOS活性.结果 30Gy组在照射后24 h核固缩百分数为(25.3±3.57)%,较0Gy组(1.95%±0.78%)有显著性差异(P<0.01);30Gy+人参皂甙Rgl 20 mol/L组在照射后24 h核圆缩百分数为(7.43±1.51)%,较30Gy组(P<0.01)及0Gy组(P<0.01)均有显著性差异.30Gy组在照射后24 h细胞培养液NOS活性为(6.46±0.95)U/ml,较0Gy组(3.20±0.70)U/ml]有显著性差异(P<0.01),30Gy+人参皂甙Rgl 20 mol/L组在照射后24hNOS活性为(3.85±0.69)U/ml,较30Gy组(P<0.01)及0Gy组(P<0.05)均有显著性差异.结论 应用人参皂甙可以通过降低X线照射后NOS活性而显著减少神经元的凋亡. 核圆缩百分数为(7.43±1.51)%,较30Gy组 P<0.01)及0Gy组(P<0.01)均有显著性差异.30Gy组在照射后24 h细胞培养液NOS活性为(6.46±0.95)U/ml,较0Gy组(3.20±0.70)U/ml]有显著性差异(P<0.01),30Gy+人参皂甙Rgl 20 mol/L组在照射后24hNOS活性为(3.85±0.69)U/ml,较30Gy组(P<0.01)及0Gy组(P<0.05)均有显著性差异.结论 应用人参皂甙可以通过降

Ginsenoside Rg1 protects rat hippocampal neurons from radiation injury by regulating NOS activity

Objective To study the protective effect of ginsenoside Rg1 on cultured rat hippocampal neurons against radiation injury and explore new therapies for preventing radiation encephalopathy. Methods Rat hippocampal neurons cultured for 12 days were subjected to a single-dose X-ray exposure of 30 Gy. 4',6-diamidino-2-phenylindole (DAPI) staining was used to detect the neuronal apoptosis and NOS activity kit utilized to evaluate NOS activity in the cells after the exposure. Results Nuclear condensation was detec...