| 重组腺病毒ADV-TK 基因的构建及其对肝癌细胞的杀伤作用 |
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| 引用本文： | 陈耿臻,韩慧,许铭炎,邓小玲.重组腺病毒ADV-TK 基因的构建及其对肝癌细胞的杀伤作用[J].南方医科大学学报,2010,30(8):1887-1889. |
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| 作者姓名： | 陈耿臻 韩慧 许铭炎 邓小玲 |
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| 作者单位： | 汕头大学医学院第二附属医院外三科，广东 汕头 515041 |
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| 基金项目： | 广东省自然科学基金，广东省科技计划项目，广东省医学科研基金 |
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| 摘 要： | 目的 探讨含胸苷激酶(TK)自杀基因的重组腺病毒(ADV-TK)对肝癌细胞的杀伤作用.方法 采用细胞内同源重组法构建出携带TK基因的ADV-TK,经PCR鉴定正确后进行扩增、纯化和滴度测定,将ADV-TK感染人肝癌细胞株SMMC-7721,MTT法检测受感染的SMMC-7721细胞被不同浓度GCV作用后的细胞存活率情况.结果 构建的重组腺病毒中带有TK基因,用相同滴度的重组腺病毒和不同浓度的GCV作用于肝癌细胞株SMMC-7721后.MTT法检测到细胞的存活率随着GCV浓度的增加而不断降低.结论 本实验构建的携带TK基因的复制缺陷型腺病毒对肝癌细胞具有明显的杀伤作用.
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| 关 键 词： | 肝癌 基因治疗 胸苷激酶基因 腺病毒载体 |
Construction of recombinant adenovirus containing TK gene and its effect against human liver cancer cells |
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| CHEN Geng-zhen,HU Hui,XU Ming-yan,DENG Xiao-ling.Construction of recombinant adenovirus containing TK gene and its effect against human liver cancer cells[J].Journal of Southern Medical University,2010,30(8):1887-1889. |
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| Authors: | CHEN Geng-zhen HU Hui XU Ming-yan DENG Xiao-ling |
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| Institution: | CHEN Geng-zhen,HU Hui,XU Ming-yan,DENG Xiao-ling Third Department of Surgery,Second Affiliated Hospital,Shantou University Medical College,Shantou 515041,China |
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| Abstract: | Objective To construct a replication-defective adenovirus containing TK gene and investigate the killing effects of TK gene against human liver cancer cells SMMC-7721. Methods The recombinant adenovirus ADV-TK was constructed using homologous recombination in the cells. SMMC-7721 cells transfected with recombined adenovirus were exposed to GCV, and the cell viability was measured by MTT assays. Results The recombinant adenovirus containing TK gene was successfully constructed. Transfection by the recombinan... |
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| Keywords: | liver cancer gene therapy thymidine kinase adenovirus vectors |
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