清热镇惊散定性定量方法研究

目的：建立清热镇惊散的定性定量方法。方法：采用薄层色谱法对方中黄连、青黛进行了定性鉴别,同时采用高效液相色谱法测定了制剂中栀子苷的含量,色谱柱为迪马C18（4.6 mm×150 mm,5μm）,流动相为乙腈-水（13∶87）,检测波长为238 nm。结果：定性鉴别薄层色谱特征明显,含量测定栀子苷在24.58~73.74μg范围内呈良好的线性关系（r=0.9996）,平均回收率为99.72%,RSD 0.98%（n=6）。结论：建立的定性定量方法可控制制剂的质量。

Study on Quality and Quantity Methods of Qingre Zhenjing Powder

Objective ： To establish the methods for the quantification and quantitation of Qingre Zhenjing power. Method： Coptidis rhizome and Indigo Naturalis were identified by TLC.The content of geniposide was determined by HPLC. HPLC method was performed on a Agilent TC-Cls （ 4.6 mm × 150mm, 5μm ） column with a mobile phase of acetonitrile -water （ 13 ： 87 ）. The wavelength of detector was 238 nm. Result： Characteristics of identification were very obvious. The linear range of geniposide was 24.58-73.74 μg （ r=0.9996 ）. The average recovery was 99.72% ; RSD was 0.98% （ n=6 ）. ConcIusion： The method can be used for the quality and quantity control of Qingre Zhenjing power.