Ad-hIL-24体外诱导喉癌细胞Hep-2的凋亡作用

目的研究腺病毒介导的人白细胞介素-24（hIL-24）基因选择性诱导喉癌细胞Hep-2凋亡的作用。方法将携带有hIL-24基因的重组复制缺陷型腺病毒（Ad-hIL-24）感染喉癌细胞Hep-2,并以人胚肺成纤维细胞WI-38为对照,采用RT-PCR法检测Hep-2、WI-38细胞中外源性hIL-24基因表达;采用四甲基偶氮唑蓝（MTT）比色法、激光扫描共聚焦显微镜（LSCM）及流式细胞术等方法检测细胞的生长和凋亡情况。结果腺病毒介导的hIL-24基因能在Hep-2细胞和WI-38细胞中表达;MTT法显示Ad-hIL-24能明显抑制喉癌细胞Hep-2的生长,LSCM观察结果表明Ad-hIL-24能促进喉癌细胞Hep-2凋亡,流式细胞术显示Hep-2细胞经Ad-hIL-24感染48h后凋亡率为18.47%,G2/M期细胞百分比为38.9%,显著高于PBS组、Ad-GFP组和WI-38细胞,差异有高度统计学意义（P〈0.01）。结论Ad-hIL-24能抑制喉癌细胞Hep-2生长和诱导Hep-2细胞凋亡,但对正常细胞无毒性作用。

Adenovirus Vector Expressing hIL-24 Induces Apoptosis in Laryngocarcinoma Cell Hep-2 in vitro

Objective To study the effect of Ad-hIL-24 in inducing apoptosis in human laryngo- carcinoma cell Hep-2. Methods The human IL-24 gene was transfected into human laryngeal cancer cell line Hep-2 and human diploid cell line WI-38 with a replication-incompetent adenovirus vector. The Ad-hIL-24 gene expression was confirmed by RT-PCR in Hep-2 cells and WI-38 cells. The cell growth and the apoptotic effect were tested by MTT assay,Hoechest 33258 staining and laser scanning confocal microscopy (LSCM) and flow cyt...