MAGE-A1,-A4基因在大肠肿瘤中的表达及临床意义

Expression and Clinical Significance of MAGE-A1, -A4 Genes in Human Colorectal Neoplasms

AIM: To explore the expression and clinical significance of MAGE-A1, MAGE-A4 genes in human colorectal neoplasm. METHODS: MAGE-A1, MAGE-A4 were detected by streptavidin-peroxidase (S-P) immunohistochemistry in 38 normal colorectal mucosal tissues, 31 colorectal adenoma tissues and 83 colorectal adenocarcinoma tissues. RESULTS: MAGE-A1 was located in cytoplasm, whereas MAGE-A4 was observed in both cytoplasm and nucleus. While hardly detected in normal colorectal mucosal tissues, their expression was significantly increased in colorectal neoplasm tissues (P<0.01). However, there was no difference between colorectal adenoma and colorectal adenocarcinoma. The expression of both genes had no relationship with sex, age, histological type, recurrence and malignant transformation of colorectal adenoma, or histological grade, depth of wall invasion and liver metastasis of colorectal adenocarcinomas. However, the expres- sion of both genes was significantly correlated with regional lymph node metastasis in colorectal adenocarcinoma (PA1<0.01, rsA1 = 0.287, PA4<0.01, rsA4 = 0.312). There was correlation between the expression of MAGE-A1 and MAGE-A4 in colorectal adenocarcinoma with regional lymph node metastasis (P<0.05, re = 0.247). CONCLUSION: MAGE-A1 and -A4 genes may play important roles in the occurrence of colorectal neoplasm and the progression of colorectal adenocarcinoma, especially the lymph nodes metastasis. So they could be regarded as two molecular markers for early diagnosis of colorectal neoplasm as well as prediction of regional lymph node metastasis of colorectal adenocarcinoma.