溶血磷脂酸诱导人卵巢上皮癌裸鼠腹腔移植瘤生长研究

目的 观察溶血磷脂酸(LPA)对卵巢上皮性癌裸鼠腹腔移植瘤生长及转移的作用.方法 建立人卵巢上皮性癌腹腔移植瘤模型,16只裸鼠随机分为2组即对照组和LPA处理组,将6×106个未经处理的卵巢上皮癌细胞株SKOV3和用LPA刺激后的SKOV3细胞分别注入2组裸鼠腹腔,待裸鼠自然死亡后,观察移植瘤大小、重量、腹腔转移灶的数目及腹腔脏器转移情况.结果 两组成瘤率均为100%,但LPA组移植瘤生长速度、重量和腹腔转移灶数目均高于对照组(P＜0.01).结论 LPA具有促进卵巢癌细胞在腹腔内增生和浸润转移的作用.

Lysophosphatidic acid promotes transplanted tumor growth of human epithelium ovarian cancer in nude mice

OBJECTIVE: To investigate effect of lysophosphatidic acid (LPA) on growth and metastasis of intraperitonally transplanted tumor in nude mice. METHODS: Sixteen nude mice randomly divided into control group and LPA treatment group, in which 6x10(6) untreated and LPA-treated human ovarian cancer cells skov3 were respectively transplanted intraperitoneally. After death of the nude mice without interventions, the weight of the transplanted tumors and number of metastatic tumors were recorded. RESULTS: The rate of tumor formation was 100%in both groups, but the treatment group showed more rapid tumor growth, greater tumor weight and more numerous metastatic tumors than the control group (P<0.01). CONCLUSION: LPA simulates the growth and promotes invasiveness of transplanted ovarian cancer cells in the peritoneal cavity of nude mie.