轮状病毒感染对新生大鼠肠上皮屏障的影响

目的探讨轮状病毒感染对大鼠肠上皮屏障的影响。方法 7日龄清洁级SD大鼠30只,随机分为对照组和轮状病毒感染组,每组15只,予以人工喂养。轮状病毒感染组在喂养的第4天(即10日龄)给予轮状病毒1×106PFU灌胃,观察其腹泻病程,并分别于感染后第1、4、7天处死,采集血液及小肠组织,观测肠黏膜形态,检测血浆D-乳酸、肠道黏蛋白mucin2、细胞间连接蛋白claudin-1表达量及肠上皮细胞凋亡。结果轮状病毒感染组较对照组大鼠的D-乳酸水平升高,以感染后第4天最显著,在第1、4、7天的差异均有统计学意义(P均<0.01);轮状病毒感染组较对照组mucin2表达量在感染后第1天增多(P<0.05),感染后第4天减少(P<0.05),第7天两组无差异(P>0.05);轮状病毒感染组较对照组claudin-1表达量在感染后第1天无差异(P>0.05),感染后第4天及第7天增加(P<0.05),以第4天最显著;轮状病毒感染组较对照组感染后第4天肠上皮细胞凋亡明显增加。结论轮状病毒感染性腹泻可能与肠上皮屏障受损有关。

Influence on intestinal epithelial barrier in rats with diarrhea of rotavirus infection

Objective To investigate the influence on intestinal epithelial barrier in rats with diarrhea of rotavirus infection.Methods Thirty 7-day-old Sprague Dawley rats of clean grade were randomly divided into control group and rotavirus infection group,fifteen in each group.Rats of both groups were fed on artificial food.Rotavirus infection group were inoculated with rotavirus of 1 × 106 PFU on day 10.On days 1,4 and 7 after infection,rats were sacrified per time point.Meanwhile,blood and the intestinal tissue were collected.The intestinal mucosal morphology,content of plasma D-lactic acid,expression of mucin2,claudin-1 and apoptosis index of intestinal epithelial cells were detected.Results Plasma D-lactic acid level was increased in rotavirus infection group than that in control group on 1,4,7 days after infection(P < 0.01),especially on day 4 after infection.The expression of mucin2 was increased in rotavirus infection group than that in control group on day 1 after infection(P < 0.05),while it was decreased in rotavirus infection group than that in control group on day 4 after infection(P < 0.05).It had no difference in both groups on day 7 after infection(P < 0.05).The expression of claudin-1 in both groups had no difference on day 1 after infection(P > 0.05).It was increased in rotavirus infection group than that in control group on day 4 and 7 after infection(P < 0.05),especially on day 4 after infection.The apoptosis of intestinal epithelial cells were obviously increased in rotavirus infection group than that in control group on day 4 after infection.Conclusions The occurrence of diarrhea of rotavirus infection may be related to the impairment of intestinal epithelial barrier.