去铁胺对新生鼠缺氧缺血性脑损伤海马DG区BrdU和nestin表达的影响

目的探讨去铁胺(DFO)对新生大鼠缺氧缺血性脑损伤(HIBD)后海马DG区5-溴-2-脱氧尿嘧啶核苷(BrdU)和巢蛋白(nestin)表达的影响。方法选用7日龄新生Wistar大鼠制作新生大鼠HIBD模型,随机分为HIBD对照组和DFO干预组,并设置假手术组。DFO干预组于脑缺氧缺血后0、24、48 h腹腔注射DFO,每次0.2 mg/g。术后4 d检测脑组织病理改变,采用间接免疫荧光组织化学技术检测海马DG区nestin、BrdU表达;于大鼠32日龄时用Morris水迷宫检测大鼠的空间学习、记忆能力。结果术后4 d,假手术组、HIBD对照组和DFO干预组大鼠的Brdu阳性细胞中位数和四分位数间距分别为(11.00,18.25)、(32.50,25.50)和(44.00,35.25),三组比较差异有统计学意义(P<0.01);nestin阳性细胞荧光强度分别为0.1279±0.0037、0.1330±0.0032、0.1365±0.0018,三组比较差异有统计学意义(P<0.01)。Morris水迷宫试验:三组大鼠的逃避潜伏期差异无统计学意义(P>0.05);假手术组、HIBD对照组、DFO治疗组大鼠的穿环指数分别为6.38±2.39、2.88±1.25和5.25±2.76,三组比较差异有统计学意义(P<0.05),两两比较,假手术组与HIBD对照组、DFO治疗组与HIBD对照组之间的差异有统计学意义(P<0.05),DFO治疗组与假手术组的差异无统计学意义(P>0.05)。结论DFO早期干预可增加新生鼠HIBD后海马DG区nestin、BrdU的表达,改善HIBD新生大鼠的空间学习记忆能力。

Effects of deferoxamine on expression of bromodeoxyuridine and nestin in the dentate gyrus region of neonatal rats with hypoxic-ischemic brain damage

Objective To investigate the effects of deferoxamine(DFO)on expression of bromodeoxyuridine(BrdU)and nestin in the dentate gyrus region of neonatal rats with hypoxic-ischemic brain damage(HIBD).Methods Wistar rats aged 7 days old were selected for HIBD model was established in the rats.All the rats were randomly divided into DFO-treated group and control group of HIBD,and sham-operated group was also set up.In DFO-treated group,DFO were injected intraperitoneally with a dose of 0.2 mg/g three times at 0,24,and 48 hours after hypoxia-ischemia(HI).Expressions of nestin and BrdU in the hippocampal dentate gyrus region were tested by indirect immunofluorescence labeling of paraffin-embedded tissues 4 days after operation and learning and memory capabilities of rats in each group were evaluated by Morris water maze at the age of 32 days.Results The number of BrdU-positive cells in hippocampal dentate gyrus region 4 days after the operation in sham-operated group,control group of HIBD and DFO-treated group was(11.00,18.25),(32.50,25.50)and(44.00,35.25),respectively.There was a significant difference in three groups(P < 0.01).The immunofluorescence integral optical density value of nestin in sham-operated group,control group of HIBD and DFO-treated group were(0.127 9 ± 0.003 7),(0.133 0 ± 0.003 2)and(0.136 5 ± 0.001 8),respectively.There was a significant difference(P < 0.01)in three groups.The latency to escape platform had no significant difference(P > 0.05)in three groups tested by Morris water maze.However,the frequency of passing through the platform in sham-operated group,control group of HIBD and DFO-treated group were(6.38 ± 2.39),(2.88 ± 1.25)and(5.25 ± 2.76),respectively.There was a significant difference(P < 0.05)in three groups.By pairwise comparison,significant differences were found between sham-operated group and control group of HIBD(P < 0.05),DFO-treated group and control group of HIBD(P < 0.05),but no difference was found between DFO-treated group and sham-operated group(P > 0.05).Conclusions Early intervention of DFO can increase the expression of BrdU and nestin in dentate gyrus region of neonatal rats with HIBD and improve the capabilities of spatial learning and memory of HIBD rats.