医院感染肺炎克雷伯菌的染色体和质粒DNA指纹图分析

目的 建立肺炎克雷伯菌重复序列聚合酶链反应技术(rep-PCR)，并应用于临床菌株流行病学调查。方法 对临床分离的39株医院感染肺炎克雷伯菌进行质粒图谱分析，并采用NaI裂解——玻璃粉吸附法提取其染色体DNA后行rep-PCR分型。结果 39株临床分离菌株产生了不同的rep-PCR带型，聚类分析显示，可分为6型，以1，2，3型为主。质粒图谱分析可将其分为4型，以1型为主，该型仅含有一种质粒。结论 (1)rep-PCR具有不需已知核酸序列，分型能力强，分辨效果好，简便快捷等优点，是进行分子流行病学的有效工具。(2)引起太和医院近两年肺炎克雷伯菌医院感染的传染源主要有3个，各型之间存在严重的交叉感染。

Rep-PCR and plasmid profile analysis for typing Klebsiella pneumoniae strains

Objective To establish the rep-PCR DNA fingerprinting technique for typing K. pneumoniae strains and apply it in the epidemiological investigation.Methods The 39 strains of K. pneumoniae were typed by plasmid profile analysis, then their chromosomal DNA were extracted and purified by NaI-lyses-glass-powder absorption method for rep-PCR. Results Different strains of K.pneumoniae showed different rep-PCR fingerprint patterns. Phylogenetic analysis showed that they could be classified into six types, mainly type 1, type 2, and type 3. Otherwise, plasmid profile analysis suggested that there were four types; mainly type 1 which contained only one plasmid.Conclusions (1) Rep-PCR DNA fingerprinting technique developed in this study is enough for epidemiological studies with its high typeability, strong discrimination, simplicity and rapidness. (2) There were three predominant K. pneumoniae transmitted between patients in Taihe Hospital during the last two years, and there might be serious cross-infection among different types.