| 高效液相色谱法测定饮料中的咖啡因 |
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| 引用本文： | 崔海精,郑彬,宁琳琪.高效液相色谱法测定饮料中的咖啡因[J].中国热带医学,2009,9(10):2058-2059. |
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| 作者姓名： | 崔海精 郑彬 宁琳琪 |
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| 作者单位： | 湛江市疾病预防控制中心,广东,湛江,524037 |
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| 摘 要： | 目的研究了高效液相色谱法直接测定饮料中咖啡因的新方法。方法采用Hypersil ODS柱，以甲醇＋乙酸铵（0．02mol／L）=15＋85作流动相，流速1．0mL/min，检测波长207nm，柱温30℃。结果6min内将咖啡因与基体分离，咖啡因含量与峰面积在10—200μg／mL范围呈线性关系，相关系数r=0．9998；回收率范围为97．4％-101．4％。结论该法准确，样品处理简单。
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| 关 键 词： | 咖啡因 饮料 高效液相色谱 |
Determination of caffeine in beverages by HPLC |
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| CUI Hai-jing,ZHENG Bin,NING Lin-qi.Determination of caffeine in beverages by HPLC[J].China Tropical Medicine,2009,9(10):2058-2059. |
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| Authors: | CUI Hai-jing ZHENG Bin NING Lin-qi |
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| Institution: | .(Zhanjiang Municipal Center for Disease Control and Prevention,Zhanjiang 524037, Guangdong,P. R.China ) |
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| Abstract: | Objeetive To establish a new method for determination of caffeine in beverages by HPLC. Methods The caffeine in beverages was determined by Hypersil ODS column using methanol-0.02 mol/L ammonium aeetae solution （15： 85） as the mobile phase at a flow rate of 1.0 mL/min and detected by VWD at 207 nm. Results Caffeine in mixture was separated within 6 minutes.The standard curve showed a direct linear correlation of the concentration with the peak area. The linear range was 10μg/mL to 200μg/mL（r=-0.9998）. Recovery rate was between 97.4% and 101.4%. Conclusion The method is accurate, simple and suitable for determination of caffeine in beverages. |
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| Keywords: | Caffeine Beverages HPLC |
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