肺鳞癌中人乳头瘤病毒与p53和Rb积聚的关系

目的探讨肺鳞癌组织中人乳头瘤病毒(humanpapillomavirus,HPV)感染与p53蛋白和Rb蛋白表达的关系并分析作用机理。方法采用地高辛标记的HPV-DNA探针,用原位杂交法检测84例肺鳞癌49例支气管黏膜慢性炎标本,组织中的HPV-DNA存在情况,并用免疫组化SABC法检测肺鳞癌组织中p53及Rb蛋白。结果HPV原位杂交阳性信号位于细胞核内,肺鳞癌HPV检出率为48.81%(41/84)、对照组黏膜慢性炎HPV检出率为6.12%(3/49),两组比较差异有统计学意义(P=0)。84例肺鳞癌中31例检出Rb蛋白表达,其中18例HPV阳性,13例HPV阴性,HPV阳性组与阴性组差异无统计学意义(P=0.2588)。84例肺鳞癌中30例检出p53蛋白表达,其中17例HPV阳性,13例HPV阴性,HPV阳性组与阴性组差异无统计学意义(P=0.3634)。结论肺鳞癌与HPV感染关系密切,肺鳞癌组织中也存在p53蛋白积聚,p53蛋白积聚与HPV感染无明显相关性,Rb蛋白积聚与HPV感染无明显相关性。

The relationship of the human papillomavirus infection and accumulation of Rb and p53 in squamous cell carcinoma of lung

Objective:To investigate the relationship between human papillomavirus (HPV) infection and the accumulation of p53 and Rb in squamous cell carcinoma (SQCC)of the lung . Methods: Eighty-four of SQCC and 49 of bronchial chronic inflammation tissues were detected by in situ hybridization for human papillomavirus using digoxingemin labelled DNA probe. The accumulation of Rb and p53 were revealed by SABC immunohistochemistry . Results: The positive signal of HPV was mainly located in the nuclei of the cells. 48.81% (41/84)of squamous cell carcinoma(SQCC) and 6.12%(6/49) of chronic inflammation tissue were positive for HPV-DNA by in situ hybridization; 31 of 84 squamous cell carcinomas were positive for Rb and no significant difference between HPV positive and HPV negative groups was found. 30 of 84 squamous cell carcinomas were positive for p53 and significant difference between HPV positive and HPV negative groups was not found. Conclusion: HPV-DNA is closely associated with SQCC,and there is accumulation of p53 and Rb in SQCC, but the relationship between HPV-DNA and accumulation of p53 and Rb was not found.