逆转录聚合酶链反应在艾滋病患者HIV-1毒株env基因的序列测定和亚型分析中的应用

目的 了解深圳地区艾滋病患者中Ⅰ型人类免疫缺陷病毒(HIV-1)毒株各亚型的感染情况，及其不同亚型对患者疾病进程的影响。方法 采用巢式逆转录聚合酶链反应(RT-PCR)法对26例艾滋病患者血浆中的HIV-1毒株的env基因片段进行扩增，并对扩增片段进行序列测定和分析，同时检测其CD4^ 细胞计数与血浆病毒载量。结果 基因系统树显示深圳地区26例艾滋病患者的HIV-1毒株env基因序列，B亚型12例，其中1例与欧美B亚型序列十分接近，11例与中国云南B亚型序列十分接近；循环重组亚型AE(CRFD1-AE)13例，均与泰国AE亚型序列十分接近。比较B、AE亚型感染患者的CD4细胞计数与病毒载量之间的差异无统计学意义。结论 深圳地区艾滋病患者中以HIV-1毒株以B亚型和循环重组亚型AE最常见，尚未观察到B、AE亚型对艾滋病进展的影响。

Genetic analysis of human immunodeficiency virus type 1 strains from AIDS patients in Shenzhen

Objective To identify subtypes of human immunodeficiency virus 1(HIV-1)strains from the AIDS patients in Shenzhen and determine whether the HIV-1 subtypes differ in disease progression.Methods HIV-1 env gene was amplified by the nest-RT-PCR from plasma obtained from 26 patients with AIDS in Shenzhen. The C2-V3 regions were sequenced to identify subtypes The plasma viral loads and CD4T lymphocyte were measured as the same time.Results Phylogenetic trees showed that the 12 AIDS patients had subtype B in which, one was close with the U.S reference strain and 11 with the Chinese Yunnan reference strain;13 AIDS patients had subtype CRF01-AE from Thailand;There were no differences in the CD4 cell count and plasma HIV-RNA levels between individuals infected with subtypes B and CRF01-AE.Conclusion Our study indicated that HIV-1 subtype B and CRF01-AE strains were present in AIDS patients in Shenzhen. There was no evidence that the subtypes of virus could determine disease progression.