转染外源性p16基因对恶性胶质瘤生长抑制作用的动物体内实验研究

目的 :探讨p16基因在体内对恶性胶质瘤的生长抑制作用。方法 :将外源性p16基因导入C6 细胞内 ,应用立体定向技术将C6细胞种植于SD大鼠尾状核头部 ,用核磁共振 (MR)扫描技术 ,动态观察颅内肿瘤生长情况。并通过免疫组化、原位杂交和细胞凋亡检测肿瘤细胞的增殖活性。结果 :转染组和治疗组大鼠生存期较对照组明显延长。治疗组肿瘤随时间的延长逐渐缩小。免疫组化显示转染组和治疗组P16蛋白表达明显增强。原位杂交和细胞凋亡检测表明 ,转染组和治疗组大鼠肿瘤细胞增殖活性降低。结论 :p16基因在体内有抑制恶性胶质瘤生长的作用 ;瘤体内注入p16cDNA质粒 脂质体复合物 ,可使肿瘤生长受到明显抑制 ,并使多数肿瘤消失

Experimental Study in vivo of Effect of Exogenously Transfected p16 on Growth of Malignant Glioma Cells

Aim:To study the effect of exogenous gene p16 on growth of malignant glioma cells in vivo .Methods:Exogenous p16 was transfected into rat malignant glioma cells (C 6), these C 6 cells were transplanted into the head of caudate nucleus of SD rats with stereotactic technology. The growth of brain tumor was dynamicly observed with magnetic resonance image (MRI). The proliferative activity of tumor cells was examined by immunohistochemical method, hybridization in situ and apoptosis.Results:The survival time of the transfection group and treatment group in rat was significantly longer than that of the control group. With the lapse of time, the tumor size of treatment group gradually became smaller and smaller. Immunohistochemistry showed the enhanced expression of p16 protein in the transfection and control groups. Hybridization in situ and apoptosis assay indicated that proliferative activity of tumor cells decreased in the transfection and treatment groups. Conclusion:The present results demonstrate that p16 gene has the same effect in vivo to inhibit malignant glioma from growth. When p16 cDNA was injected into tumors, it would inhibit these tumors from development and make most of them to disappear