杜仲对糖尿病大鼠扑捉行为和阴茎组织神经传导通路的影响

目的:探讨杜仲改善勃起功能的药效和病理学机制。方法:雄性糖尿病(DM)大鼠30只随机分为3组:A组(10只,DM大鼠赋形剂灌胃组)、B组(10只,DM大鼠西地那非灌胃组)、C组(10只,DM大鼠杜仲灌胃组)及10只正常对照组大鼠(赋形剂灌胃,D组);灌胃4周后观察4组大鼠扑捉行为,透射电镜检查阴茎组织有髓神经纤维超微特征;用免疫组化二步法显示阴茎组织中神经元型一氧化氮合酶(nNOS)的表达。结果:与A组比较,C组大鼠扑捉次数显著增多(P<0.05),阴茎组织中nNOS表达显著增强(P<0.001)。透射电镜显示:A组大鼠阴茎组织有髓神经纤维排布失序,部分变性、板层分离形成透明空泡或网络状,C组大鼠有髓神经纤维排列规整,板层结构清晰。结论:杜仲可通过减轻有髓神经的损伤、增强阴茎组织中nNOS表达改善ED。

The Effects of Eucommia Ulmoides Oliv on Catching Action of Diabetic Rats and Myelinated Nerve Fibers in Penile Tissues

Objective: To explore the pharmacodynamic and pathological mechanism of eucommia ulmoides oliv in improving erectile function.Methods: Thirty male diabetic rats were randomly divided into three groups: group A(n=10,escipient group),group B(n=10,sidenafil group),group C(n=10,eucommia ulmoides oliv group) and group D(n=10,the normal control group).After gavage for four weeks,the catching behaviors of all rats were observed,and ultrastructure of myelinated nerve fibers in penile tissue was examined by transmission electron microscope.The expression of neuronal nitric oxide synthase(nNOS) in penile tissues was examined by two steps immunohistochemistry method.Results: Compared with group A, catching frequency of the rats in group C was notably increased(P<(0.05))and the expression of nNOS in penile tissue was significantly(P<(0.001)).The examination by transmission electron microscope showed that in the rats' penile tissue of group A,myelinated nerve fibers were irregularly arranged and partially degenerated,and myelin sheaths lamella were splited and exhibited vacuoles or network forms.In group C,there were regular arrangements of myelinated nerve fibers,in which the formation of lamella was clear.Conclusion: By remitting the impairement of myelinated nerve fibers and enhancing the expression of nNOS in penile tissue,eucommia ulmoides oliv can improve erectile function of diabetic rats.