自体心包瓣“内皮化”的实验研究

目的 探讨有利于自体心包瓣内皮化的处理方法。方法 将体外培养小牛主动脉内皮细胞接种于不同方法处理的自体牛心包表面。采用＾３Ｈ－ＴｄＲ摄取量测定、扫描电镜及放射免疫法观察不同处理方法对接种后内皮细胞生长、前列环素（ＰＧＩ２）合成量和细胞内ｃＡＭＰ含量的影响。结果 三种不同浓度戊二醛短时间（１０ｍｉｎ）处理的心包表面内皮细胞均可生长,其中以体积分数为０．２％戊二种不同浓度戊二醛短时间（１０ｍｉｎ）处理

Experimental study on endothelialization of autologous pericardial heart valves in vitro

Objective To investigate a method of autologous pericardial heart valves (APHV) treatment beneficial to endothelialization. Methods Bovine aortic endothelium cells (BAEC) were cultured and seeded in autologous pericardial pieces treated with different methods in vitro. The influence of different treatment on cellular growing, amount of 3H TdR incorporation and PGI 2 releasing, contents of intracellular cAMP was studied by means of transmission electron microscopy, 3H TdR incorporation experimentation and radioimmunity method. Results The BAEC seeded in bovine pericardial pieces treated with three different concentrations of GA 10 minutes could alive. In the group with method of treatment with 0.2% GA, the growing of BAEC was best, the cellular density and the amount of 3H TdR incorporation at different time spots were higher than that of the other groups and it did not enhance the abnormal synthesis of cAMP and PGI 2 in BAEC. Conclusion The pericardium treated with 0.2% GA 10 minutes has little toxicity to endothelium cell, which indicates that this method is beneficial to endothelialization of APHV postoperatively.