黄芪注射液在大鼠肠缺血再灌注肝损伤中的应用实验

目的：探究黄芪注射液在大鼠肠缺血再灌注肝损伤（intestinal ischemia-reperfusion，IIR）中的应用情况及对Bcl-2 相关X 蛋白（Bcl-2 associated X protein，Bax）、B 淋巴细胞瘤-2（B-cell lymphoma-2，Bcl-2）表达水平的影响。方法：将9 只成年健康雄性Wistar 大鼠随机分为3 组，分别为假手术组（Sham 组）、模型组（IIR 组）和黄芪注射液组（黄芪组），每组3 只大鼠，3 组按照再灌注时间（1、3、6 h）划分为3 个不同时相（每一个时相均为1只大鼠），实验结束后取大鼠肝左叶作为实验标本，利用MIAS-2000 医学图像分析系统及免疫组化法检测全部标本Bax、Bcl-2 表达水平，利用缺口末端标注技术（TUNEL）检测肝组织中肝细胞凋亡指数（apoptosis index，AI）。结果：与Sham 组相比，IIR 组与黄芪组3 个不同时相Bax、Bcl-2 表达水平均明显提高（P<0.05），IIR 组3个不同时相Bcl-2/Bax 降低，黄芪组3 个不同时相Bcl-2/Bax 水平均降低（P<0.05）。与IIR 组对比，黄芪组再灌注1、3、6 h 3 个不同时相Bcl-2/Bax、Bcl-2 都显著提高，Bax 水平降低（P<0.05）。同组中不同时相间对比无明显差异（P>0.05）。与Sham 组相比，IIR 组与黄芪组再灌注1、3、6 h 3 个不同时相AI 均显著提高（P<0.05）。与IIR组对比，黄芪组3 个不同时相AI 都显著降低（P<0.05）。IIR 组与黄芪组同组中不同时相间对比具有明显差异（P<0.05），而且再灌注6 h 最高。结论：大鼠发生IIR 后可导致肝损伤，而且Bcl-2、Bax 表达水平提高，黄芪注射液的作用机制可能在于调节两种表达，缓解肝细胞凋亡，具有肝保护效果。

Application of Astragalus Injection in Intestinal Ischemia-Reperfusion Injury in Rats

Objective：To investigate the application of Astragalus injection in intestinal ischemia-reperfusion liver injury in rats and its influence on the expression levels of Bax and Bcl-2. Methods：9 healthy adult male Wistar rats were randomly divided into 3 groups：Sham group，model group （IIR group） and Astragalus injection group （Astragalus group），with 3 rats in each group. The 3 groups were divided into 3 different phases（each time phase was 1 rats） according to the time of reperfusion（ 1，3，6 h）. After the end of the experiment，the expression levels of Bax and Bcl-2 in the left lobe of rat liver were taken as the experimental standard，and the expression levels of Bax and Bcl-2 in all specimens were detected by MIAS-2000 medical image analysis and immunohistochemical method，and the apoptosis index（AI） of liver tissues was detected by the TUNEL technique. Results：Compared with Sham group，the expression levels of Bax and Bcl-2 in three different phases of IIR group and Huangqi group were significantly increased（P<0.05），while those of Bcl-2/Bax and Bcl-2/Bax in three different phases of IIR group andAstragalus group were decreased （P<0.05）. Compared with the IIR group，the reperfusion of bcl-2/Bax and Bcl-2 in the astragalus group at 1，3 and 6 h was significantly increased，while the level of Bax was decreased （P<0.05）. There was no significant difference between different phases in the same group （P>0.05）. Compared with Sham group，AI of IIR group and Huangqi group were significantly improved at different phases of reperfusion at 1，3 and 6 h （P<0.05）. Compared with IIR group，AI of astragalus group in three different phases was significantly reduced （P<0.05）. There were significant differences between IIR group and Astragalus group in different phases （P<0.05），and reperfusion was the highest for 6 h. Conclusion：IIR can lead to liver injury in rats，and the expression levels of Bcl-2 and Bax are increased. The mechanism of action of Astragalus injection may be to regulate the two expressions，alleviate the apoptosis of liver cells，and have a protective effect on the liver.