前列腺癌中微管相关蛋白1轻链3的表达及其临床意义研究

目的 检测前列腺癌（PCa）组织中微管相关蛋白1轻链3（MAP1LC-3）基因和蛋白表达的变化，探讨其相关的临床意义。方法 收集2016年5月至2019年2月本院泌尿外科需手术治疗的PCa患者96例，取肿瘤组织标本和癌旁正常前列腺组织标本用于研究。荧光定量PCR检测组织MAP1LC-3基因表达水平。免疫组化染色检测组织中MAP1LC-3蛋白表达水平。结果 PCa患者癌组织中MAP1LC-3基因的2^-△△Ct值为（0.375±0.065），显著高于癌旁正常前列腺组织的（0.116±0.024）（P<0.01）。PCa组织中MAP1LC-3蛋白的AOD值为（0.351±0.046），显著高于癌旁正常前列腺组织的（0.082±0.013）（P<0.01）。PCa组织中MAP1LC-3基因2^-△△Ct值和蛋白AOD值在Ⅲ、Ⅳ期患者显著高于Ⅰ、Ⅱ期患者（P<0.01），N1期患者显著高于N0期患者（P<0.01），Gleason评分>7分患者显著高于Gleason评分≤7分患者（P<0.01），PSA>20 ng/mL患者显著高于PSA≤20 ng/mL患者（P<0.01）。结论 PCa组织中MAP1LC-3基因和蛋白高表达，高表达的MAP1LC-3可能参与了PCa的发生、侵袭和转移过程。

significance of detecting microtubule associated protein 1 light chain 3 in prostate cancer

Objective  To detect the clinical significance of MAP1LC-3 in prostate cancer. Methods  From May 2016 to February 2019, 96 patients with prostate cancer were enrolled in this study. The adjacent normal tissues were used as controls. The mRNA expression of MAP1LC-3 was detected by real time PCR analysis. The protein expression of MAP1LC-3 was detected by immunohistochemistry stains. Results  The 2^-△△Ct value of MAP1LC-3 in prostate cancer tissue was（0.375±0.065）, which was higher than that of（0.116±0.024） in adjacent normal tissues(P<0.01). The AOD value of MAP1LC-3 protein in prostate cancer tissue was（0.351±0.046）, which was higher than that of（0.082±0.013） in adjacent normal tissues(P<0.01). The 2^-△△Ct and AOD value MAP1LC-3 in Ⅲ and Ⅳ stage, which was higher than that  in Ⅰ and Ⅱ stage(P<0.01). The 2^-△△Ct  and AOD value MAP1LC-3 in N1 stage,which was higher than that in N0 stage(P<0.01). The 2^-△△Ct  and AOD value MAP1LC-3 in Gleason scores>7,which was higher than that in Gleason scores≤7(P<0.01). The 2^-△△Ct and AOD value MAP1LC-3 in PSA>20 ng/mL,which was higher than that in PSA≤20 ng/mL(P<0.01). Conclusions  High expression of MAP1LC-3 may be involved in the occurrence, invasion and metastasis of prostate cancer.