| 秋花独蒜兰化学成分及其生物活性研究 |
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| 引用本文： | 李玉鹏,陈兴龙,袁盛兴,陈亚娟,张荣平. 秋花独蒜兰化学成分及其生物活性研究[J]. 昆明医科大学学报, 2023, 44(3): 1-6. DOI: 10.12259/j.issn.2095-610X.S20230312 |
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| 作者姓名： | 李玉鹏 陈兴龙 袁盛兴 陈亚娟 张荣平 |
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| 作者单位： | 1.昆明医科大学药学院暨云南省天然药物药理重点实验室，云南 昆明 650500 |
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| 基金项目： | 国家自然科学基金资助项目 （81960666）；云南省自然科学基金资助项目（ 2019FA033）；云南省创新团队项目 （202005AE160004）；云南省“云岭学者项目” （YNWR-YLXZ-2019-019 ） |
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| 摘 要： | 目的 研究秋花独蒜兰化学成分，发现其活性成分。方法 秋花独蒜兰经95%乙醇提取、硅胶柱层析、半制备HPLC和Sephadex LH-20柱层析进行分离纯化，波谱分析（核磁共振氢谱、碳谱、和质谱）确定结构；应用MTT法，对部分化合物进行体外抗肿瘤活性筛选。结果 从秋花独蒜兰分离鉴定10个化合物，分别为：1, 7-dihydroxy-2, 5-dimethoxyphenanthrene(1), 2, 7-dihydroxy-1, 5-dime-thoxyphenanthrene(2),confusarin (3),4,7-dihydroxy-2-dimethoxy-9,10-dihydrophe-nanthrene (4),pleionesin B (5),pleionesin C (6),flavanthrin (7),2,2′-dihydroxy-5,5′，7,7′-tetramethoxy-9,9′，10,10′-tetrahydro-3,3′-biphenanthrene (8),6,6′，7,7′-tetrahy--droxy-2,2′，4,4′-tetramethoxy-8,8...
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| 关 键 词： | 秋花独蒜兰 化学成分 生物活性 |
| 收稿时间： | 2022-11-16 |
Chemical Compounds Isolated from Pleione maculata and Their Bioactive Activity |
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| Affiliation: | 1.School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunnan 6505002.School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicine Utilization，Yunnan University of Chinese Medicine，Kunming Yunnan 6505003.Dept. of Ultrasound，the First People’s Hospital of Qujing City，Qujing Yunnan 6550004.School of Rehabilitation，Kunming Medical University，Kunming Yunnan 650500，China |
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| Abstract: | Objective To study the chemical compounds from the medicinal plants of Pleione maculata and find its bioactive compounds. Methods The compounds were extracted by alcohol （95%） and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis （1H NMR, 13CNMR and EIMS）. The antitumor activity of the compounds was studied by MTT assay in vitro. Results Ten compounds were obtained and identified as 1, 7-dihydroxy-2, 5 -dimethoxyphenanthrene （ 1 ）, 2, 7-dihydroxy-1, 5-dimethoxyphenanthrene （ 2 ）, confusarin （ 3 ）, 4, 7-dihydroxy-2-dimethoxy-9, 10-dihydrophenanthrene （ 4 ）, pleionesin B （ 5 ）, pleionesin C （ 6 ）, flavanthrin （ 7 ）, 2, 2′-dihydroxy-5, 5′, 7, 7′-tetramethoxy-9, 9′, 10, 10′-tetrahydro-3, 3′-biphenanthrene （ 8 ）, 6, 6′, 7, 7′-tetrahy- -droxy-2, 2′, 4, 4′-tetramethoxy -8, 8′-biphenanthrene （ 9 ）, 1, 3′, 5′, 7-tetrahydroxy-4, 7′ -dimethoxy-9, 9′, 10, 10′-tetrahydro-2, 2′- biphenanthrene （1 0 ）; Compounds 3, 7～10 were tested for the inhibitory activity of human lung cancer cell lines （A549）, human breast cancer drug-sensitive cell lines （MCF-7/S）, and human ovarian cancer cell lines （SKOV-3）. The results of anti-tumor activity test of compounds showed that 10 showed good inhibitory activity on three cell lines. Conclusion All compounds were isolated from this plant for the first time. Compound 10 has good inhibitory activity against three tumor cell lines, and with IC50 of 2.45, 6.83, 4.23 μM, respectively. |
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