一测多评法测定六神丸中5种蟾毒配基类成分含量

目的：建立一测多评法测定六神丸中蟾毒配基类成分含量的分析方法，并验证此方法在六神丸中的可行性和适应性。方法：采用高效液相色谱法，以华蟾酥毒基（S）为内参物，建立日蟾毒它灵（A）、蟾毒它灵（B）、蟾毒灵（C）和脂蟾毒配基（D）的相对校正因子。分别采用外标法和一测多评法测定六神丸中5种蟾毒配基类成分的含量，并比较测定值和计算值之间的差异。结果：六神丸中蟾毒配基类成分间的相对校正因子分别为f_A/S=1.06，f_B/S=0.896，f_C/S=1.09，f_D/S=0.914，一测多评法的计算值与外标法的实测值的相对误差小于1%。结论：以华蟾酥毒基为内参物建立的相对校正因子准确、可行，一测多评法可用于六神丸中蟾毒配基类成分的质量评价。

Simultaneous determination of five bufadienolides in Liushen pills by QAMS

OBJECTIVE To establish a analytical method of multi-components with a single-marker (QAMS), to simultaneously determine the contents of five bufadienolides in Liushen pills, and evaluate the adaptation and application of QAMS method in the evaluation of Liushen pills. METHODS Cinobufagin was chosen as the internal reference substance. The relative correlation factors (RCF) of gamabufotalin (A), bufotalin (B), bufalin (C) and resibufogenin (D) to cinobufagin (S) were calculated and established. The contents of these five bufadienolides were determined by the external standard method and QAMS respectively. The QAMS method was validated through comparison of the results obtained by the two different methods. RESULTS RCFs of gamabufotalin(A), bufotalin(B), bufalin(C) and resibufogenin(D) to cinobufagin(S) in Liushen pills were 1.06, 0.896, 1.09 and 0.914, respectively. The relative error between the calculated value and the measured value of the QAMS was less than 1%. CONCLUSION The RCF established in the QAMS method with cinobufagin as the internal reference substance is accurate and feasible. The QAMS method can be used for the quality evaluation of multiple bufadienolides in Liushen pills.