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| 组织蛋白酶B联合TRAIL诱导胃癌BGC-823细胞凋亡的作用研究 | |
| 引用本文： | 查道德,涂龙霞,吴文涌. 组织蛋白酶B联合TRAIL诱导胃癌BGC-823细胞凋亡的作用研究[J]. 包头医学院学报, 2023, 39(1): 4-7. DOI: 10.16833/j.cnki.jbmc.2023.01.002 |
| 作者姓名： | 查道德 涂龙霞 吴文涌 |
| 作者单位： | 1.淮北职业技术学院医学系, 安徽淮北 235000; 2.安徽省第二人民医院普外科 |
| 基金项目： | *安徽省重点研究与开发计划项目人口健康专项(202104j07020005);安徽省高校自然科学研究重点课题项目(KJ2018A0712);合肥市关键共性技术研发和重大科技成果工程化项目(2021YL001) |
| 摘 要： | 目的:观察组织蛋白酶B(CTSB)联合TRAIL诱导胃癌BGC-823细胞凋亡的作用。方法:BGC-823细胞传代培养。采用MTT法检测细胞增殖情况，流式细胞仪检测细胞凋亡率以及免疫印迹法(Western blot)检测Cathepsin B蛋白表达。结果:TRAIL对BGC-823细胞增殖有抑制作用，对细胞凋亡有促进作用(P<0.05); Cathepsin B抑制剂浓度越高，细胞凋亡率越低(P<0.05);Western blot结果表明，Cathepsin B抑制剂浓度越高，Cathepsin B表达越少(P<0.05)。结论:Cathepsin B和TRAIL联合使用有协同诱导胃腺癌BGC-823细胞凋亡作用。 |
| 关 键 词： | 组织蛋白酶B 肿瘤坏死因子 凋亡配体 BGC-823细胞 细胞凋亡 |
| 收稿时间： | 2022-05-31 |
Role of cathepsin B combining with TRAIL on inducing apoptosis in gastric cancer BGC-823 cells |
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| ZHA Daode,TU Longxia,WU Wenyong. Role of cathepsin B combining with TRAIL on inducing apoptosis in gastric cancer BGC-823 cells[J]. Journal of Baotou Medical College, 2023, 39(1): 4-7. DOI: 10.16833/j.cnki.jbmc.2023.01.002 | |
| Authors: | ZHA Daode TU Longxia WU Wenyong |
| Affiliation: | 1. Department of Medicine, Huaibei Vocational and Technical College, Huaibei 235000, China; 2. Department of General Surgery, Anhui Second People's Hospital |
| Abstract: | Objective: To observe the apoptosis of gastric cancer BGC-823 cells induced by cathepsin B (CTSB) combined with TRAIL. Methods: BGC-823 cells were routinely subcultured. The proliferation of BGC-823 cells was detected with MTT Cell Proliferation Assay Kit. The apoptosis rate was detected by flow cytometry (FCM) and the expression level of cathepsin B protein was detected with Western blot. Results: TRAIL could inhibit the proliferation of BGC-823 cells and promote apoptosis(P＜0.05). The higher concentration of cathepsin B inhibitor could lead to the lower apoptosis rate(P＜0.05). Western blot results showed that the higher concentration of cathepsin B inhibitor would cause the lower expression of cathepsin B(P＜0.05). Conclusion: Cathepsin B combining with TRAIL could synergistically induce apoptosis of gastric cancer BGC-823 cells. |
| Keywords: | Cathepsin B TRAIL BGC-823 cell Apoptosis |
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