β1、β2肾上腺素能受体在口腔鳞状细胞癌组织中的表达及其临床意义

目的 探讨β1肾上腺素能受体（β1 adrenergic receptors，β1-AR）和β2肾上腺素能受体（β2 adrenergic receptors，β2-AR）在口腔鳞状细胞癌组织中的表达及其临床意义。方法 收集2017年7月至2018年7月在广西医科大学附属口腔医院口腔颌面外科手术切除的口腔鳞癌组织及相应癌旁组织60例，并收集同期于本院就诊的15例非口腔鳞癌患者的口腔正常黏膜上皮组织。采用RT-qPCR及Western blot法检测β1-AR和β2-AR mRNA及蛋白在组织中的表达，采用Cox回归分析其与临床病理特征及预后的关系。结果 β1-AR、β2-AR蛋白及β2-AR mRNA在口腔鳞状细胞癌组织中的相对表达量均高于癌旁组织（P＜0.01）及正常口腔上皮组织（P＜0.05）。β1-AR、β2-AR mRNA和蛋白表达水平均与临床分期有关，其中β2-AR表达水平还与淋巴结转移及术后复发有关（均P<0.05）。β2-AR高表达组患者1 年、3 年总生存率及无进展生存率低于β2-AR低表达组（χ2=3.945，P=0.047；χ2=9.286，P=0.002），β1-AR表达水平与患者总生存期、无进展生存期无关。校正潜在的混杂因素后，多因素Cox回归显示β2-AR蛋白低表达是影响总生存期的保护因素（HR=0.149，95%CI：0.028~0.785，P=0.025）。结论 β1-AR、β2-AR在口腔鳞状细胞癌组织中高表达，β2-AR高表达患者预后较差，可能是口腔鳞状细胞癌潜在的预后评估指标。

Expression and clinical significance of β1 and β2 adrenergic receptors in oral squamous cell carcinoma tissues

Objective To investigate the expression of β1 adrenergic receptors(β1-AR) and β2 adrenergic receptors(β2-AR) in oral squamous cell carcinoma tissues and their clinical significance. Methods A total of 60 cases of squamous cell carcinoma tissues and corresponding para-carcinoma tissues, resected by the oral and maxillofacial surgery of Guangxi Medical University College of Stomatology from July 2017 to July 2018, were collected, and the normal oral mucosal epithelial tissues of 15 non-squamous cell carcinoma patients during the same period were also collected. RT-qPCR and Western blot were used to detect the expression of β1-AR and β2-AR mRNA and protein, and Cox regression was used to analyze their relationship with clinicopathological characteristics and prognosis. Results The relative expression levels of β1-AR, β2-AR proteins and β2-AR mRNA in oral squamous cell carcinoma tissues were higher than those of para-carcinoma tissues(P<0.01) and normal oral mucosal epithelial tissues(P＜0.05). The expression levels of β1-AR, β2-AR mRNA and proteins were correlated with clinical stage, and the expression level of β2-AR was also correlated with lymph node metastasis and postoperative recurrence(both P<0.05). The 1-year, 3-year overall survival rate and progression-free survival rate in the β2-AR high expression group were lower than those in the β2-AR low expression group(χ2=3.945, P=0.047; χ2=9.286, P=0.002), but β1-AR expression level was not correlated with patients′overall survival and progression-free survival. After adjusting for potential confounding factors, multivariable Cox regression showed that the low protein expression of β2-AR was a protective factor for overall survival(HR=0.149, 95%CI: 0.028-0.785, P=0.025). Conclusions β1-AR and β2-AR are highly expressed in oral squamous cell carcinoma tissues.  Patients with high expression of β2-AR have a poor prognosis and may be a potential prognostic indicator for oral squamous cell carcinoma.